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Paracoccus niistensis sp. nov., isolated from forest soil, India

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Abstract

A Gram-negative, non-motile, catalase-positive and oxidase-positive, aerobic bacterium designated as NII-0918T was isolated from soil sample in Western ghat forest, India. 16S rRNA gene sequence analysis showed that strain NII-0918T belongs to the subclass α-Proteobacteria, being related to the genus Paracoccus, and sharing highest sequence similarity with Paracoccus chinensis NBRC 104937T (99.4%), Paracoccus marinus NBRC 100640T (97.3%), Paracoccus koreensis Ch05T (97.1%) and Paracoccus kondratievae GBT (97.0%). Other members of Paracoccus showed below 97.0% similarity. The DNA–DNA hybridization values between these four strains and NII-0918T were 44.7, 28, 32 and 41%, respectively. The major fatty acids of strain NII-0918T were summed feature 7 (C18:1 ω7c/ω 9t/ω 12t) (83.0%) and C18:0 (12.5%). Ubiquinone Q-10 was detected as the major respiratory quinone. The G+C content of genomic DNA of NII-0918T was 66.6 mol%. On the basis of physiological, morphological, chemotaxonomical and DNA–DNA hybridization data, it is proposed that strain NII-0918T should be placed as a novel species, for which we propose Paracoccus niistensis sp. nov. The type strain is NII-0918T (CCTCC AA 209055T = NCIM 5340T = KCTC 22789T).

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Acknowledgment

The authors would like to thank CSIR Task force network programme on Exploration of India’s Rich Microbial Diversity (NWP 0006) for providing the financial support.

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Authors and Affiliations

  1. Biological Oceanography Division, National Institute of Oceanography, Dona Paula, Panaji, 403004, Goa, India

    Syed G. Dastager

  2. National Institute for Interdisciplinary Science and Technology (CSIR), Trivandrum, 695019, India

    C. K. Deepa & Ashok Pandey

  3. Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, People’s Republic of China

    Wen-Jun Li & Shu-Kun Tang

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  1. Syed G. Dastager
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  2. C. K. Deepa
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  3. Wen-Jun Li
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  4. Shu-Kun Tang
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  5. Ashok Pandey
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Correspondence to Syed G. Dastager.

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Dastager, S.G., Deepa, C.K., Li, WJ. et al. Paracoccus niistensis sp. nov., isolated from forest soil, India. Antonie van Leeuwenhoek 99, 501–506 (2011). https://doi.org/10.1007/s10482-010-9515-4

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