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Bacillus zhanjiangensis sp. nov., isolated from an oyster in South China Sea

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Abstract

A novel Gram-stain-positive, motile, catalase- and oxidase-positive, endospore-forming, aerobic, rod-shaped bacterium, designated strain JSM 099021T, was isolated from an oyster collected from Naozhou Island in the South China Sea. Growth occurred with 0–15% (w/v) NaCl (optimum 2–4%) and at pH 6.0–10.0 (optimum pH 7.5) and at 10–45°C (optimum 30–35°C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant respiratory quinone was menaquinone 7 (MK-7) and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major cellular fatty acids were anteiso-C15:0, anteiso-C17:0, iso-C15:0 and iso-C16:0. The genomic DNA G + C content was 39.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 099021T belongs to the genus Bacillus, and was most closely related to the type strains of Bacillus halmapalus (sequence similarity 99.0%), Bacillus horikoshii (98.4%) and Bacillus cohnii (98.0%). The combination of phylogenetic analysis, DNA–DNA hybridization, phenotypic characteristics and chemotaxonomic data supported the proposal that strain JSM 099021T represents a new species of the genus Bacillus, for which the name Bacillus zhanjiangensis sp. nov. is proposed. The type strain was JSM 099021T (=DSM 23010T = KCTC 13713T).

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Acknowledgments

This work was supported by grants from the National Basic Research Program of China (2010CB833800), National Natural Science Foundation of China (NSFC) (30970007), Key Program of Shanghai Agrobiological Gene Center (SAGC200903) and Key Laboratory of Ecotourism’s Application Technology of Hunan Province (SL2010Z01). We are grateful to Mr Qi-Hui Chen and Zhu-Xiang Liu for their excellent technical assistance.

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Authors and Affiliations

  1. Key Laboratory of Ecotourism’s Application Technology of Hunan Province, College of Biology and Environmental Sciences, Jishou University, Jishou, 416000, People’s Republic of China

    Yi-Guang Chen, Jian-Wu He, Jian-Qing Xiao & Hong-Yi Zhu

  2. College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, 330045, People’s Republic of China

    Song-Ping Hu

  3. The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Shu-Kun Tang & Wen-Jun Li

  4. Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, People’s Republic of China

    Wen-Jun Li

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  1. Yi-Guang Chen
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Correspondence to Song-Ping Hu.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 099021T is HM460884.

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Chen, YG., Hu, SP., Tang, SK. et al. Bacillus zhanjiangensis sp. nov., isolated from an oyster in South China Sea. Antonie van Leeuwenhoek 99, 473–480 (2011). https://doi.org/10.1007/s10482-010-9510-9

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