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Antonie van Leeuwenhoek

, Volume 99, Issue 2, pp 329–339 | Cite as

Optimization of bioethanol production during simultaneous saccharification and fermentation in very high-gravity cassava mash

  • Bao Yingling
  • Yan Zongcheng
  • Wang Honglin
  • Chen LiEmail author
Original Paper

Abstract

Hydrolysis and fermentation conditions for production of ethanol from very high-gravity cassava mash by Saccharomyces cerevisiae during simultaneous saccharification and fermentation (SSF) processing were optimized using a statistical methodology. During the first part of the study, Placket–Burman design (PBD) was used to study 19 factors that could potentially influence ethanol production. Gravity, particle size, initial pH, and fermentation temperature were identified as key factors that significantly increased final ethanol concentration. The main and interaction effects of these factors were subsequently evaluated based on a quadratic equation generated by central composite design (CCD) using response-surface methodology (RSM). Under the optimized very high-gravity conditions, the final ethanol concentration obtained from experiment increased from 8.21% (wt.%) to 15.03% (wt.%) and was in good agreement with model prediction. By employing two other commercial Saccharomyces strains, similar results were obtained under the same optimized condition. Therefore, we conclude that final ethanol concentration, ethanol productivity (V P/max), glucose utilization (Y G/s, Y P/s), and fermentation efficiency (η f) were enhanced or maintained under the optimized condition of 40% gravity, 390 μm particle size, initial pH 5.5, and 27°C fermentation temperature.

Keywords

Saccharomyces cerevisiae Ethanol production Very high gravity Simultaneous saccharification and fermentation Placket–Burman design Response-surface methodology 

Notes

Acknowledgments

This research was supported by the Ministry of Science and Technology International Cooperation Project 18-509J, Research Found of Science and Technology Bureau for the International Cooperation Project of Guangdong Province 2009B050800011.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Bao Yingling
    • 1
  • Yan Zongcheng
    • 1
  • Wang Honglin
    • 1
  • Chen Li
    • 1
    Email author
  1. 1.School of Chemistry and Chemical EngineeringSouth China University of TechnologyGuangzhouPeople’s Republic of China

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