Abstract
A novel anamorphic yeast strain, A1-01T, belonging to the genus Rhodotorula was isolated from a plant in Taiwan and analysed morphologically, physiologically and phylogenetically. Neither ballistoconidia nor sexual reproduction was observed. Sequence analysis of the 26S rRNA gene and the ITS region indicate that Rhodosporidium sphaerocarpum is the most closely related species, with 14 and 24 nucleotide substitutions, respectively. The novel species differed physiologically from R. sphaerocarpum in its ability to assimilate ethylamine and cadaverine, its inability to assimilate ethanol and nitrite. From these comparative analyses, the following novel yeast species is proposed: Rhodotorula taiwanensis sp. nov. with the type strain of A1-01T (BCRC 23118T = CBS 11729T).
Similar content being viewed by others
References
Barnett JA, Payne RW, Yarrow D (2000) Yeasts: characteristics and identification, 3rd edn. Cambridge University Press, Cambridge
Biswas SK, Yokoyama K, Nishimura K, Miyaji M (2001) Molecular phylogenetics of the genus Rhodotorula and related basidiomycetous yeasts inferred from the mitochondrial cytochrome b gene. Int J Syst Evol Microbiol 51:1191–1199
Fell JW, Boekhout T, Fonseca A, Scorzetti G, Statzell-Tallman A (2000) Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351–1371
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
Felsenstein J (2004) PHYLIP (phylogenetic inference package), version 3.6. Distributed by the author. Department of Genome Sciences and Department of Biology, University of Washington, Seattle
Fonseca A, Scorzetti G, Fell JW (2000) Diversity in the yeast Cryptococcus albidus and related species as revealed by ribosomal DNA sequence analysis. Can J Microbiol 46:7–27
Gadanho M, Sampaio JP (2002) Polyphasic taxonomy of the basidiomycetous yeast genus Rhodotorula: Rh. glutinis sensu stricto and Rh. dairenensis comb nov. FEMS Yeast Res 2:47–58
Hamamoto M, Nagahama T, Tamura M (2002) Systematic study of basidiomycetous yeasts evaluation of the ITS regions of rDNA to delimit species of the genus Rhodosporidium. FEMS Yeast Res 2:409–413
Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 73:331–371
Libkind D, Brizzio S, Ruffini A, Gadanho M, van Broock M, Paulo Sampaio J (2003) Molecular characterization of carotenogenic yeasts from aquatic environments in Patagonia, Argentina. Antonie Van Leeuwenhoek 84:313–322
Nagahama T, Hamamoto M, Horikoshi K (2006) Rhodotorula pacifica sp. nov., a novel yeast species from sediment collected on the deep-sea floor of the north-west pacific ocean. Int J Syst Evol Microbiol 56:95–299
Page RDM (1996) TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
Suh SO, Takashhima M, Nakase T (1996) Phylogenetic study of the anamorphic basidiomycetous yeasts Rhodotorula lactosa and R. minuta, and related taxa based on 18S ribosomal DNA sequences. J Gen Appl Microbiol 42:1–6
Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M et al (eds) PCR protocols: a guide to methods and applications. Academic Press, Orlando, pp 315–322
Yarrow D (1998) Methods for the isolation, maintenance and identification of yeasts. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 77–100
Acknowledgements
We thank Drs. S. K. Chen, C. C. Liao and G. F. Yuan (Food Industry Research and Development Institute, Taiwan, ROC) for their encouragement. This research was supported by Ministry of Economic Affairs, ROC (project no. 99-EC-17-A-R7-0525) for FIRDI, and the Chang Gung Medical Research Foundation and National Science Council for Dr. P. W. Hsieh.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Huang, CH., Lee, FL., Tien, CJ. et al. Rhodotorula taiwanensis sp. nov., a novel yeast species from a plant in Taiwan. Antonie van Leeuwenhoek 99, 297–302 (2011). https://doi.org/10.1007/s10482-010-9489-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10482-010-9489-2