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Bacillus deserti sp. nov., a novel bacterium isolated from the desert of Xinjiang, China

Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

A Gram-positive, rod-shaped, motile and spore-forming bacterium, designated ZLD-8T, was isolated from a desert soil sample collected from Xinjiang Province in north-west China, and subjected to a polyphasic taxonomic analysis. This isolate grew optimally at 30°C and pH 7.0. It grew with 0–4% NaCl (optimum, 0–1%). Comparative 16S rRNA gene sequence analysis showed that strain ZLD-8T was closely related to members of the genus Bacillus, exhibiting the highest 16S rRNA gene sequence similarity to Bacillus kribbensis DSM 17871T (98.0%). The levels of 16S rRNA gene sequence similarity with respect to other Bacillus species with validly published names were less than 96.3%. The DNA G + C content of strain ZLD-8T was 40.1 mol%. The strain contained MK-7 as the predominant menaquinone. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids (>5% of total fatty acids) were anteiso-C15:0 (39.56%), iso-C14:0 (25.69%), C16:1 ω7c alcohol (10.13%) and iso-C15:0 (5.27%). These chemotaxonomic results supported the affiliation of strain ZLD-8T to the genus Bacillus. However, low DNA–DNA relatedness values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation of strain ZLD-8T from recognized Bacillus species. On the basis of the polyphasic evidence presented, strain ZLD-8T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus deserti sp. nov. is proposed. The type strain is ZLD-8T (=CCTCC AB 207173T = KCTC 13246T).

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References

  • Ausubel FW, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1995) Preparation and analysis of DNA. In: Current protocols in molecular biology. Wiley, New York

  • Bae SS, Lee JH, Kim SJ (2005) Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of the Ayu Trough. Int J Syst Evol Microbiol 55:1211–1215

    Article  CAS  PubMed  Google Scholar 

  • Buczolits S, Denner EBM, Vybiral D, Wieser M, Kämpfer P, Busse HJ (2002) Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov. Int J Syst Evol Microbiol 52:445–456

    CAS  PubMed  Google Scholar 

  • Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK, Lim YW (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261

    Article  CAS  PubMed  Google Scholar 

  • Claus D, Berkeley RCW (1986) Genus Bacillus Cohn, 1872. In: Sneath PHA, Mair NS, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 2. Williams & Wilkins, Baltimore, pp 1105–1139

    Google Scholar 

  • Demharter W, Hensel R (1989) Bacillus thermocloaceae sp. nov., a new thermophilic species from sewage sludge. Syst Appl Microbiol 11:272–276

    Google Scholar 

  • Derekova A, Mandeva R, Kambourova M (2008) Phylogenetic diversity of thermophilic carbohydrate degrading bacilli from Bulgarian hot springs. World J Microbiol Biotechnol 24:1697–1702

    Article  CAS  Google Scholar 

  • Doetsch RN (1981) Determinative methods of light microscopy. In: Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR, Phillips GH (eds) Manual of methods for general bacteriology. American Society for Microbiology, Washington, DC, pp 21–33

    Google Scholar 

  • Ezaki T, Hashimoto Y, Habuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229

    Article  Google Scholar 

  • Felsenstein J (2006) PHYLIP (Phylogeny Inference Package), v3.66. Department of Genome Sciences, University of Washington, Seattle

    Google Scholar 

  • Heyrman J, Vanparys B, Logan NA, Balcaen A, Rodríguez-Díaz M, Felske A, De Vos P (2004) Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands. Int J Syst Evol Microbiol 54:47–57

    Article  CAS  PubMed  Google Scholar 

  • Heyrman J, Logan NA, Rodríguez-Díaz M, Scheldeman P, Lebbe L, Swings J, Heyndrickx M, De Vos P (2005) Study of mural painting isolates, leading to the transfer of ‘Bacillus maroccanus’ and ‘Bacillus carotarum’ to Bacillus simplex, emended description of Bacillus simplex, re-examination of the strains previously attributed to ‘Bacillus macroides’ and description of Bacillus muralis sp. nov. Int J Syst Evol Microbiol 55:119–131

    Article  CAS  PubMed  Google Scholar 

  • Ko KS, Oh WS, Lee MY, Lee JH, Lee H, Peck KR, Lee NY, Song JH (2006) Bacillus infantis sp. nov. and Bacillus idriensis sp. nov., isolated from a patient with neonatal sepsis. Int J Syst Evol Microbiol 56:2541–2544

    Article  CAS  PubMed  Google Scholar 

  • Kovács N (1956) Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703–704

    Article  PubMed  Google Scholar 

  • Lane DJ (1991) 16S-23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 125–175

    Google Scholar 

  • Lim JM, Jeon CO, Lee JR, Park DJ, Kim CJ (2007) Bacillus kribbensis sp. nov., isolated from a soil sample in Jeju, Korea. Int J Syst Evol Microbiol 57:2912–2916

    Article  CAS  PubMed  Google Scholar 

  • Lin YC, Uemori K, de Briel DA, Arunpairojana V, Yokota A (2004) Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae. Int J Syst Evol Microbiol 54:1669–1676

    Article  CAS  PubMed  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  • MIDI (1999) Sherlock microbial identification system operating manual, version 3.0. MIDI, Inc., Newark, DE

  • Palmisano MM, Nakamura LK, Duncan KE, Istock CA, Cohan FM (2001) Bacillus sonorensis sp. nov., a close relative of Bacillus licheniformis, isolated from soil in the Sonoran Desert, Arizona. Int J Syst Evol Microbiol 51:1671–1679

    CAS  PubMed  Google Scholar 

  • Pettersson B, de Silva S, Uhlèn M, Priest FG (2000) Bacillus siralis sp. nov., a novel species from silage with a higher order structural attribute in the 16S rRNA genes. Int J Syst Evol Microbiol 50:2181–2187

    CAS  PubMed  Google Scholar 

  • Pichinoty F, Asselineau J, Mandel M (1984) Charactérisation biochemique de Bacillus benzoevorans sp. nov., une nouvelle espèce filamenteuse, engainée et mésophile dégradant divers acides aromatiques et phénols. Ann Microbiol/Inst Pasteur 135B:209–217 (in French)

    Article  CAS  Google Scholar 

  • Rheims H, Frühling A, Schumann P, Rohde M, Stackebrandt E (1999) Bacillus silvestris sp. nov., a new member of the genus Bacillus that contains lysine in its cell wall. Int J Syst Bacteriol 49:795–802

    Article  PubMed  Google Scholar 

  • Rhodes ME (1958) The cytology of Pseudomonas spp. as revealed by a silver-plating staining method. J Gen Microbiol 18:639–648

    CAS  PubMed  Google Scholar 

  • Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654

    Google Scholar 

  • Staneck JL, Roberts GD (1974) Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231

    CAS  PubMed  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetic analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  PubMed  Google Scholar 

  • Tiago I, Pires C, Mendes V, Morais PV, da Costa MS, Veríssimo A (2006) Bacillus foraminis sp. nov., isolated from a non-saline alkaline groundwater. Int J Syst Evol Microbiol 56:2571–2574

    Article  CAS  PubMed  Google Scholar 

  • Tindall BJ (1990) A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130

    CAS  Google Scholar 

  • Vaishampayan P, Probst A, Krishnamurthi S, Ghosh S, Osman SR, McDowall A, Rukmani A, Mayilraj S, Venkateswaran K (2009) Bacillus horneckiae sp. nov., isolated from a clean room where the Phoenix spacecraft was assembled. Int J Syst Evol Microbiol. doi:10.1099/ijs.0.008979-0

  • Venkateswaran K, Kempf M, Chen F, Satomi M, Nicholson W, Kern R (2003) Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are -radiation resistant. Int J Syst Evol Microbiol 53:165–172

    Article  CAS  PubMed  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE et al (1987) International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  • Xie CH, Yokota A (2003) Phylogenetic analysis of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349

    Article  CAS  PubMed  Google Scholar 

  • Yumoto I, Hirota K, Yamaga S, Nodasaka Y, Kawasaki T, Matsuyama H, Nakajima K (2004) Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids. Int J Syst Evol Microbiol 54:1997–2001

    Article  CAS  PubMed  Google Scholar 

  • Zhang L, Wang Y, Dai J, Tang Y, Yang Q, Luo X, Fang C (2009) Bacillus korlensis sp. nov., a moderately halotolerant bacterium isolated from a sand soil sample in China. Int J Syst Evol Microbiol 59:1787–1792

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by the R & D Infrastructure and Facility Development Program from the Ministry of Science and Technology of the People’s Republic of China (Grant No.2005DKA21208) and NWSUAF (Z111020910) China.

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Authors and Affiliations

  1. College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, People’s Republic of China

    Lei Zhang

  2. College of Life Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Lei Zhang, Yang Wang, Jun Dai & Cheng-Xiang Fang

  3. State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau of Northwest A&F University, Institute of Soil and Water Conservation of CAS&MWR, Yangling, Shaanxi, 712100, People’s Republic of China

    Gao-Lin Wu

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  1. Lei Zhang
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  2. Gao-Lin Wu
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  5. Cheng-Xiang Fang
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Correspondence to Cheng-Xiang Fang.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain ZLD-8T is GQ465041.

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Zhang, L., Wu, GL., Wang, Y. et al. Bacillus deserti sp. nov., a novel bacterium isolated from the desert of Xinjiang, China. Antonie van Leeuwenhoek 99, 221–229 (2011). https://doi.org/10.1007/s10482-010-9479-4

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