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Nocardiopsis terrae sp. nov., a halophilic actinomycete isolated from saline soil

Abstract

A Gram-positive, moderately halophilic, facultatively alkaliphilic, catalase- and oxidase-positive, obligately aerobic, filamentous actinomycete strain, designated YIM 90022T, was isolated from saline soil collected from the Qaidam Basin, north-west China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolate was a member of the genus Nocardiopsis and the sequence similarities between the isolate and the type strains of members of the genus Nocardiopsis were in the range of 95.1–98.7%. Phenotypic and chemotaxonomic properties of this organism also indicated that strain YIM 90022T was a member of the genus Nocardiopsis. The strain grew well on most of the media tested, producing yellow-white to deep brown substrate mycelium and white aerial mycelium. Light gray to deep brown diffusible pigments were produced. The substrate mycelium was well developed and fragmented with age; the aerial mycelium produced long, straight to flexuous spore chains with non-motile, smooth-surfaced, rod-shaped spores on them. The strain grew in the presence of 1–15% (w/v) total salts (optimum, 3–5%) and at pH 6.0–10.5 (optimum, pH 8.5) and 10–45°C (optimum, 30°C). Whole-cell hydrolysates of strain YIM 90022T contained meso-diaminopimelic acid and no diagnostic sugars. The predominant menaquinones were MK-10(H4), MK-9(H8), MK-10(H6) and MK-10(H8). Polar lipids comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylmethylethanolamine. The major cellular fatty acids were iso-C16:0, anteiso-C17:0, 10-methyl-C18:0 and 10-methyl-C17:0. The DNA G + C content of strain YIM 90022T was 71.5 mol%. The combination of phylogenetic analysis, DNA–DNA relatedness data, phenotypic characteristics and chemotaxonomic data supported the suggestion that strain YIM 90022T represents a new species of the genus Nocardiopsis, for which the name Nocardiopsis terrae sp. nov. is proposed. The type strain is YIM 90022T (=CCTCC AA 208011T =KCTC 19431T).

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Acknowledgements

This work was supported by grants from the National Basic Research Program of China (2010CB833800), National Natural Science Foundation of China (NSFC) (30970007), Key Projects of International Cooperation (2007DFB31620) and Yunnan Provincial Natural Science Foundation (Nos. 2009AC017 and 2009DA002). We are grateful to Mr Yun Chen and Mr Ke Huang for their excellent technical assistance.

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Correspondence to Yi-Guang Chen or Wen-Jun Li.

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM 90022T is DQ387958.

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Chen, YG., Zhang, YQ., Tang, SK. et al. Nocardiopsis terrae sp. nov., a halophilic actinomycete isolated from saline soil. Antonie van Leeuwenhoek 98, 31–38 (2010). https://doi.org/10.1007/s10482-010-9425-5

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  • DOI: https://doi.org/10.1007/s10482-010-9425-5

Keywords

  • Nocardiopsis terrae sp. nov.
  • Halophilic
  • Saline soil