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Diversity and identification of methanogenic archaea and sulphate-reducing bacteria in sediments from a pristine tropical mangrove

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Abstract

Mangrove sediments are anaerobic ecosystems rich in organic matter. This environment is optimal for anaerobic microorganisms, such as sulphate-reducing bacteria and methanogenic archaea, which are responsible for nutrient cycling. In this study, the diversity of these two functional guilds was evaluated in a pristine mangrove forest using denaturing gradient gel electrophoresis (DGGE) and clone library sequencing in a 50 cm vertical profile sampled every 5.0 cm. DGGE profiles indicated that both groups presented higher richness in shallow samples (0–30 cm) with a steep decrease in richness beyond that depth. According to redundancy analysis, this alteration significantly correlated with a decrease in the amount of organic matter. Clone library sequencing indicated that depth had a strong effect on the selection of dissimilatory sulphate reductase (dsrB) operational taxonomic units (OTUs), as indicated by the small number of shared OTUs found in shallow (0.0 cm) and deep (40.0 cm) libraries. On the other hand, methyl coenzyme-M reductase (mcrA) libraries indicated that most of the OTUs found in the shallow library were present in the deep library. These results show that these two guilds co-exist in these mangrove sediments and indicate important roles for these organisms in nutrient cycling within this ecosystem.

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Acknowledgements

The authors sincerely express their thanks and gratitude to CNPq—Conselho Nacional de Pesquisa e Desenvolvimento Tecnológico and FAPESP—Fundação de Amparo à Pesquisa do Estado de São Paulo for the financial support of the projects No. 474455/2007-6 and No. 2006/06700-0, respectively. We also would like to thank the fellowships given by: (a) CNPq to R.G. Taketani, Caio Yoshiura and S.M.Tsai (PQ), and (b) FAPESP to F. D. Andreote and A.C.F. Dias.

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Authors and Affiliations

  1. Molecular and Cellular Biology, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, 303 Centenário Av. 13400-970, Piracicaba, SP, Brazil

    Rodrigo Gouvêa Taketani, Caio Augusto Yoshiura, Armando Cavalcante Franco Dias & Siu Mui Tsai

  2. Laboratory of Environmental Microbiology, EMBRAPA Environment, Jaguariuna, SP, Brazil

    Armando Cavalcante Franco Dias & Fernando Dini Andreote

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  1. Rodrigo Gouvêa Taketani
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  2. Caio Augusto Yoshiura
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  3. Armando Cavalcante Franco Dias
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  4. Fernando Dini Andreote
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  5. Siu Mui Tsai
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Correspondence to Rodrigo Gouvêa Taketani.

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Taketani, R.G., Yoshiura, C.A., Dias, A.C.F. et al. Diversity and identification of methanogenic archaea and sulphate-reducing bacteria in sediments from a pristine tropical mangrove. Antonie van Leeuwenhoek 97, 401–411 (2010). https://doi.org/10.1007/s10482-010-9422-8

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  • DOI: https://doi.org/10.1007/s10482-010-9422-8

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