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Strains nodulating Lupinus albus on different continents belong to several new chromosomal and symbiotic lineages within Bradyrhizobium

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Abstract

In this work we analysed different chromosomal and symbiotic markers in rhizobial strains nodulating Lupinus albus (white lupin) in several continents. Collectively the analysis of their rrs and atpD genes, and 16S-23S intergenic spacers (ITS), showed that they belong to at least four chromosomal lineages within the genus Bradyrhizobium. Most isolates from the Canary Islands (near to the African continent) grouped with some strains isolated on mainland Spain and were identified as Bradyrhizobium canariense. These strains are divided into two ITS subgroups coincident with those previously described from isolates nodulating Ornithopus. The remaining strains isolated on mainland Spain grouped with most isolates from Chile (American continent) forming a new lineage related to Bradyrhizobium japonicum. The strains BLUT2 and ISLU207 isolated from the Canary Islands and Chile, respectively, formed two new lineages phylogenetically close to different species of Bradyrhizobium depending on the marker analyzed. The analysis of the nodC gene showed that all strains nodulating L. albus belong to the biovar genistearum; nevertheless they form four different nodC lineages of which lineage C is at present exclusively formed by L. albus endosymbionts isolated from different continents.

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Acknowledgment

This work was supported by the Regional Government of Castilla y León (Grants GR49 and CSI04A06) and partially by Grant BOS2003-05858 from the Ministerio de Ciencia y Tecnología of the Spanish Government. A. Valverde was supported by a postdoctoral JAE-Doc (CSIC) contract and R. Rivas by a postdoctoral contract of “Ramón y Cajal” program.

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  1. Departamento de Microbiología y Genética, Lab. 209, Edificio Departamental de Biología, Universidad de Salamanca, Campus M. Unamuno, 37007, Salamanca, Spain

    Encarna Velázquez, Raúl Rivas, Víctor Gomis, Inne Gantois, Pedro F. Mateos & Eustoquio Martínez-Molina

  2. Instituto de Recursos Naturales y Agrobiología, IRNASA-CSIC, Salamanca, Spain

    Angel Valverde, Álvaro Peix & José M. Igual

  3. Departamento de Microbiología y Biología Celular, Universidad de La Laguna, Tenerife, Spain

    Milagros León-Barrios

  4. Laboratorium voor Microbiologie, Vakgroep Biochemie en Microbiologie, Universiteit Gent K.L, Ledeganckstraat 35, B-9000, Ghent, Belgium

    Anne Willems

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  1. Encarna Velázquez
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  2. Angel Valverde
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Correspondence to Encarna Velázquez.

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Velázquez, E., Valverde, A., Rivas, R. et al. Strains nodulating Lupinus albus on different continents belong to several new chromosomal and symbiotic lineages within Bradyrhizobium . Antonie van Leeuwenhoek 97, 363–376 (2010). https://doi.org/10.1007/s10482-010-9415-7

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