Abstract
A novel anamorphic Cryptococcus species is described, which was isolated in New Delhi (India) from decaying wood of a tree trunk hollow of Ficus religiosa. On the basis of sequence analysis of the D1/D2 domains of the 26S rRNA gene and the internally transcribed spacer (ITS)-1 and ITS-2 region sequences, the isolate belonged to the Cryptococcus albidus cluster (Filobasidiales, Tremellomycetes) and was closely related to Cryptococcus saitoi, Cryptococcus cerealis and Cryptococcus friedmannii with 98% sequence identity. Phenotypically, the species differed from C. saitoi with respect to growth temperature (up to 37oC), presence of a thin capsule, ability to grow in the absence of vitamins, and inability to assimilate citrate and ethylamine. With respect to C. friedmannii, it differed in growth temperature, ability to assimilate lactose, raffinose, l-rhamnose, myo-inositol, and inability to utilize citrate. Furthermore, our isolate also differed from C. cerealis in growth temperature, presence of capsule and inability to assimilate l-sorbose. In view of the above phenotypic differences and unique rDNA sequences, we consider that our isolate represents a new species of Cryptococcus, and therefore, a new species, Cryptococcus randhawai is proposed for this taxon. The type strain J11/2002 has been deposited in the culture collection of the Centraalbureau voor Schimmelcultures (CBS10160) and CABI Biosciences (IMI 393306).
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References
Ahmad S, Khan Z, Mustafa AS, Khan ZU (2002) Semi-nested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification. J Clin Microbiol 40:2483–2489
Ahmad S, Khan Z, Mokaddas E, Khan ZU (2004) Isolation and molecular identification of Candida dubliniensis from non-human immunodeficiency virus-infected patients in Kuwait. J Med Microbiol 53:633–637
Ahmad S, Al-Mahmeed M, Khan ZU (2005) Characterization of Trichosporon species isolated from clinical specimens in Kuwait. J Med Microbiol 54:639–646
Asadzadeh M, Al-Sweih NA, Ahmad S, Khan ZU (2008) Antifungal susceptibility of clinical Candida parapsilosis isolates in Kuwait. Mycoses 51:318–323
Barnett JA, Payne RW, Yarrow D (2000) Yeasts: characteristics and identification, 3rd edn. Cambridge University Press, Cambridge
Fell JW, Statzel-Tallman A (1998) Cryptococcus Vuillemin. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic Study, 4th edn edn. Elsevier, Amsterdam, pp 742–767
Fell JW, Boekhout T, Fonseca A, Scorzetti G, Statzell-Tallman A (2000) Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351–1371
Fonseca A, Scorzetti G, Fell JW (2000) Diversity in the yeast Cryptococcus albidus and related species as revealed by ribosomal DNA sequence analysis. Can J Microbiol 46:7–27
Granados DP, Castañeda E (2005) Isolation and characterization of Cryptococcus neoformans varieties recovered from natural sources in Bogotá, Colombia, and study of ecological conditions in the area. Microb Ecol 49:282–290
Herzberg M, Fischer R, Titze A (2002) Conflicting results obtained by RAPD-PCR and large-subunit rDNA sequences in determining and comparing yeast strains isolated from flowers: a comparison of two methods. Int J Syst Evol Microbiol 52:1423–1433
Hong SG, Lee KH, Bae KS (2002) Diversity of yeasts associated with natural environment in Korea. J Microbiol 40:55–62
Khan ZU, Ahmad S, Mokaddas E, Chandy R, Cano J, Guarro J (2008) Actinomucor elegans var kuwaitiensis isolated from the wound of a diabetic patient. Antonie van Leeuwenhoeck 94:343–352
Kidd SE, Hagen F, Tscharke RL, Huynh M, Bartlett KH, Fyfe M, Macdougall L, Boekhout T, Kwon-Chung KJ, Meyer W (2004) A rare genotype of Cryptococcus gattii caused the cryptococcosis outbreak on Vancouver Island (British Columbia, Canada). Proc Natl Acad Sci USA 101:17258–17263
Kumar S, Dudley J, Nei M, Tamura K (2008) MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306
Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large-subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73:331–371
Lazera MS, Cavalcanti MA, Trilles L, Nishikawa MM, Wanke B (1998) Cryptococcus neoformans var. gattii—evidence for a natural habitat related to decaying wood in a pottery tree hollow. Med Mycol 36:119–122
Nei M, Kumar S (2000) Molecular evolution and phylogenetics. Oxford University Press, New York
Paliwal DK, Randhawa HS (1978) Evaluation of a simplified Guizotia abyssinica seed medium for differentiation of Cryptococcus neoformans. J Clin Microbiol 7:346–348
Passoth V, Andersson A-C, Olstorpe M, Theelen B, Boekhout T, Schnurer J (2009) Cryptococcus cerealis sp nov. a psychrophilic yeast species isolated from fermented cereals. Antonie Van Leeuwenhoek 96:635–643
Pfeiffer TJ, Ellis DH (1992) Environmental isolation of Cryptococcus neoformans var gattii from Eucalyptus tereticornis. J Med Vet Mycol 30:407–408
Randhawa HS, Mussa AY, Khan ZU (2001) Decaying wood in tree trunk hollows as a natural substrate for Cryptococcus neoformans and other yeast-like fungi of clinical interest. Mycopathologia 151:63–69
Randhawa HS, Kowshik T, Khan ZU (2003) Decayed wood of Syzygium cumini and Ficus religiosa living trees in Delhi/New Delhi metropolitan area as natural habitat of Cryptococcus neoformans. Med Mycol 41:199–209
Sanfelice F (1894) Ueber eine fur Tiere pathogene Sprosspilzart und uber die morphologisch Uebereinstimmung, welche sie bei ihrem Voorkommen in den Geweben mit den vermeintlichen Krebscoccidien zeigt. Centralb Bakt Parasitenkunde 1. Abt. 17:113–118
Staib F, Thielke C, Randhawa HS, Senska M, Kulins G (1972) Colonisation of dead plants by Cryptococcus neoformans. Zentralbl Bakteriol [Orig A] 222:15–25
Acknowledgments
The authors would like to thank Prof. Dr. Walter Gams and Prof. Justo Hernandez for their kind assistance with the Latin diagnosis of the strain J11/2002, and to Zaiba Khan and Leena Joseph for technical assistance. This study was supported in part by Kuwait University Research Administration grant MI 118.
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Khan, Z.U., Ahmad, S., Hagen, F. et al. Cryptococcus randhawai sp. nov., a novel anamorphic basidiomycetous yeast isolated from tree trunk hollow of Ficus religiosa (peepal tree) from New Delhi, India. Antonie van Leeuwenhoek 97, 253–259 (2010). https://doi.org/10.1007/s10482-009-9406-8
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DOI: https://doi.org/10.1007/s10482-009-9406-8