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Survival of native Pseudomonas in soil and wheat rhizosphere and antagonist activity against plant pathogenic fungi

Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

Survival of Pseudomonas sp. SF4c and Pseudomonas sp. SF10b (two plant-growth-promoting bacteria isolated from wheat rhizosphere) was investigated in microcosms. Spontaneous rifampicin-resistant mutants derived from these strains (showing both growth rate and viability comparable to the wild-strains) were used to monitor the strains in bulk soil and wheat rhizosphere. Studies were carried out for 60 days in pots containing non-sterile fertilized or non-fertilized soil. The number of viable cells of both mutant strains declined during the first days but then became established in the wheat rhizosphere at an appropriate cell density in both kinds of soil. Survival of the strains was better in the rhizosphere than in the bulk soil. Finally, the antagonism of Pseudomonas spp. against phytopatogenic fungi was evaluated in vitro. Both strains inhibited the mycelial growth (or the resistance structures) of some of the phytopathogenic fungi tested, though variation in this antagonism was observed in different media. This inhibition could be due to the production of extracellular enzymes, hydrogen cyanide or siderophores, signifying that these microorganisms might be applied in agriculture to minimize the utilization of chemical pesticides and fertilizers.

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Acknowledgments

The authors thank J. Raaijmakers and M. Espinosa-Urgel for providing reference strains of Pseudomonas and M. Colombo (INTA) for the strain Rhizoctonia. We are also grateful to Dr. Donald F. Haggerty, a retired career investigator and native English speaker, for editing the final version of the manuscript. S Fischer and E Jofré are members of the Scientific Researcher Career-CONICET (National Council of Technological Researchs). P. Cordero is recipient of a doctoral fellowship from CONICET-Agencia Córdoba Ciencia. This research was supported by SECYT of the Universidad Nacional de Río Cuarto, Picto-Agencia Nacional de Promoción Científica y Tecnológica (Argentina).

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Authors and Affiliations

  1. Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36-Km 601, CP 5800, Río Cuarto, Córdoba, Argentina

    Sonia E. Fischer, Edgardo C. Jofré, Paula V. Cordero & Gladys B. Mori

  2. Facultad de Farmacia, Universidad San Pablo CEU, Boadilla del Monte, Madrid, España

    Francisco J. Gutiérrez Mañero

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  1. Sonia E. Fischer
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  2. Edgardo C. Jofré
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  3. Paula V. Cordero
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  4. Francisco J. Gutiérrez Mañero
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  5. Gladys B. Mori
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Correspondence to Sonia E. Fischer.

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Fischer, S.E., Jofré, E.C., Cordero, P.V. et al. Survival of native Pseudomonas in soil and wheat rhizosphere and antagonist activity against plant pathogenic fungi. Antonie van Leeuwenhoek 97, 241–251 (2010). https://doi.org/10.1007/s10482-009-9405-9

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  • DOI: https://doi.org/10.1007/s10482-009-9405-9

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