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Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR

Antonie van Leeuwenhoek volume 96, Article number: 441 (2009) Cite this article

Abstract

The increased occurrence of Salmonella occurrence in local indigenous vegetables and poultry meat can be a potential health hazards. This study is aimed to detect the prevalence of twenty different virulence factors among Salmonella enterica strains isolated from poultry and local indigenous vegetables in Malaysia via an optimized, rapid and specific multiplex PCR assay. The assay encompasses a total of 19 Salmonella pathogenicity islands genes and a quorum sensing gene (sdiA) in three multiplex reaction sets. A total of 114 Salmonella enterica isolates belonging to 38 different serovars were tested. Each isolate in under this study was found to possess up to 70% of the virulence genes tested and exhibited variable pathogenicity gene patterns. Reproducibility of the multiplex PCR assay was found to be 100% and the detection limit of the optimized multiplex PCR was tested with lowest detectable concentration of DNA 0.8 pg μl−1. This study demonstrated various Salmonella pathogenicity island virulence gene patterns even within the same serovar. This sets of multiplex PCR system provide a fast and reliable typing approach based on Salmonella pathogenicity islands, thus enabling an effective monitoring of emerging pathogenic Salmonella strains as an additional tool in Salmonella surveillance studies.

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Acknowledgments

The authors thank the Department of Chemistry, Malaysia for collaboration and providing the Salmonella isolates in this study. This study was supported by Fundamental Research Grant Scheme (04-01-07-098) provided from the Ministry of Higher Education, Malaysia.

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Affiliations

  1. Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia

    Chai-Hoon Khoo, Yoke-Kqueen Cheah, Learn-Han Lee, Jiun-Horng Sim & Sabrina Sukardi

  2. Department of Chemistry Malaysia, Section of Biotechnology, Unit Microbiology, Jalan Sultan, 46661, Petaling Jaya, Selangor, Malaysia

    Noorzaleha Awang Salleh

  3. Department of Pathology, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia

    Shiran Mohd Sidik

  4. Department of Food Science, Faculty of Food Science and Technology, University Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia

    Son Radu

Authors
  1. Chai-Hoon Khoo
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  2. Yoke-Kqueen Cheah
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  3. Learn-Han Lee
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  5. Noorzaleha Awang Salleh
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  6. Shiran Mohd Sidik
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  7. Son Radu
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Correspondence to Yoke-Kqueen Cheah.

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Khoo, CH., Cheah, YK., Lee, LH. et al. Virulotyping of Salmonella enterica subsp. enterica isolated from indigenous vegetables and poultry meat in Malaysia using multiplex-PCR. Antonie van Leeuwenhoek 96, 441 (2009). https://doi.org/10.1007/s10482-009-9358-z

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  • DOI: https://doi.org/10.1007/s10482-009-9358-z

Keywords

  • Virulotyping
  • Salmonella enterica
  • Salmonella pathogenicity islands (SPIs)
  • Multiplex PCR