Abstract
The genetic structure of populations of the out-crossing haplontic yeast species Metschnikowia lochheadii was investigated. The species is associated with floricolous beetles in Central America and Hawaii. The objective was to determine whether sexual reproduction is prevalent and to what extent the geographic distribution of genotypes can be viewed as historical. The genetic markers examined include the mating type (h + or h −) and nine polymorphic DNA loci. The data were used to assess population structuring based on F ST and linkage disequilibrium and the distribution of alleles using parsimony haplotype networks. In Central America, M. lochheadii is subdivided into sexually active demes between which gene flow is limited. Isolates from five Hawaiian islands had identical haplotypes, confirming that the species has undergone a founder effect concomitant with the recent import of a nitidulid beetle into the archipelago.
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Acknowledgments
We acknowledge funding from the Natural Science and Engineering Research Council of Canada and the issuance of collection or research permits from the Costa Rican Ministry of the Environment and the United States National Park Service. We are indebted to R. Blanco, J. M. Bowles, M. M. Chavarria-Diaz, C. P. Ewing, D. H. Janzen, D. Koscinski, and W. T. Starmer for assistance in the field or in the laboratory. Funding to DHJ and WTS from the National Science Foundation is also acknowledged. Thanks are extended to GE Healthcare Canada for their cooperation. We appreciate comments on the manuscript by N. Keyghobadi, B. Neff, G. J. Thompson, A. Wardlaw, and several anonymous reviewers.
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Lachance, MA., Lawrie, D., Dobson, J. et al. Biogeography and population structure of the Neotropical endemic yeast species Metschnikowia lochheadii . Antonie van Leeuwenhoek 94, 403–414 (2008). https://doi.org/10.1007/s10482-008-9258-7
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DOI: https://doi.org/10.1007/s10482-008-9258-7