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Nocardioides islandiensis sp. nov., isolated from soil in Bigeum Island Korea

Abstract

A novel actinobacterium designated as MSL-26T was isolated from soil in Bigeum Island Korea. A polyphasic study was undertaken to establish the taxonomic position of isolate MSL-26T. Strain MSL-26T was found to have chemical and morphological characteristics similar to Nocardioides. The strain grew optimally at pH 7·5 and 28°C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MSL-26T forms a distinct line of descent within the radiation enclosed by the genus Nocardioides. The cell wall of strain MSL-26T contained LL-2, 6-diaminopimelic acid. The principal menaquinone was MK-8 (H4). The phospholipids detected were diphosphatidylglycerol, phosphatidylglycerol and some unidentified lipids. C18:1 w7c (50.38%) was the major fatty acid. The DNA G + C content of strain MSL-26T was 71.4 mol%. The 16S rRNA gene sequence of strain MSL-26T shares the highest sequence similarity with Nocardioides kribbensis KCTC 19038T (95.78%) and Nocardioides aquaticus DSM 11439T (95.52%). Based on the morphological, physiological, biochemical and chemotaxonomical data presented in this study, strain MSL-26T should be classified as a novel species, for which the name Nocardioides islandiensis sp. nov. is proposed. The type strain is MSL-26T (=KCTC 19275=DSM 19321T)

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Acknowledgement

This work was supported by the 21C Frontier Microbial Genomics and Application Centre program Korean Ministry of Science & Technology (MOST) and Korea Foundation for International Cooperation of Science & Technology (KICOS) through a grant provided by MOST in Global Partnership Program (No. M60602000001-06E0200-00100), Republic of Korea.

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Correspondence to Chang-Jin Kim.

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Dastager, S.G., Lee, JC., Ju, YJ. et al. Nocardioides islandiensis sp. nov., isolated from soil in Bigeum Island Korea. Antonie van Leeuwenhoek 93, 401–406 (2008). https://doi.org/10.1007/s10482-007-9217-8

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  • DOI: https://doi.org/10.1007/s10482-007-9217-8

Keywords

  • Nocardioides islandiensis sp. nov
  • Polyphasic taxonomy
  • 16S rRNA