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Overexpression of ADH1 and HXT1 genes in the yeast Saccharomyces cerevisiae improves the fermentative efficiency during tequila elaboration

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Abstract

This work assessed the effect of the overexpression of ADH1 and HXT1 genes in the Saccharomyces cerevisiae AR5 strain during fermentation of Agave tequilana Weber blue variety must. Both genes were cloned individually and simultaneously into a yeast centromere plasmid. Two transformant strains overexpressing ADH1 and HXT1 individually and one strain overexpressing both genes were randomly selected and named A1, A3 and A5 respectively. Overexpression effect on growth and ethanol production of the A1, A3 and A5 strains was evaluated in fermentative conditions in A. tequilana Weber blue variety must and YPD medium. During growth in YPD and Agave media, all the recombinant strains showed lower cell mass formation than the wild type AR5 strain. Adh enzymatic activity in the recombinant strains A1 and A5 cultivated in A. tequilana and YPD medium was higher than in the wild type. The overexpression of both genes individually and simultaneously had no significant effect on ethanol formation; however, the fermentative efficiency of the A5 strain increased from 80.33% to 84.57% and 89.40% to 94.29% in YPD and Agave medium respectively.

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Acknowledgements

This research was supported by the Consejo Nacional de la Ciencia y Tecnología de México (CONACYT, project 41148).

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Authors and Affiliations

  1. Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico

    Melesio Gutiérrez-Lomelí, Luis Alberto Cira-Chávez & Carlos Pelayo-Ortiz

  2. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Unidad de Biotecnología, Av. Normalistas No. 800, Col. Colinas de la Normal, C.P. 44270, Guadalajara, Jalisco, Mexico

    Melesio Gutiérrez-Lomelí, Luis Alberto Cira-Chávez & Jose de Jesús Ramírez-Córdova

  3. Instituto de Investigación en Biología Experimental. Facultad de Química, Universidad de Guanajuato, Guanajuato, Mexico

    Juan Carlos Torres-Guzmán & Gloria Angélica González-Hernández

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  1. Melesio Gutiérrez-Lomelí
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  2. Juan Carlos Torres-Guzmán
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  3. Gloria Angélica González-Hernández
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  4. Luis Alberto Cira-Chávez
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  5. Carlos Pelayo-Ortiz
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  6. Jose de Jesús Ramírez-Córdova
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Correspondence to Jose de Jesús Ramírez-Córdova.

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Gutiérrez-Lomelí, M., Torres-Guzmán, J.C., González-Hernández, G.A. et al. Overexpression of ADH1 and HXT1 genes in the yeast Saccharomyces cerevisiae improves the fermentative efficiency during tequila elaboration. Antonie van Leeuwenhoek 93, 363–371 (2008). https://doi.org/10.1007/s10482-007-9213-z

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  • DOI: https://doi.org/10.1007/s10482-007-9213-z

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