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Streptomyces tritolerans sp. nov., a novel actinomycete isolated from soil in Karnataka, India

Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

A Gram-positive, nonmotile, moderately halophilic, alkali and thermotolerant strain designated DAS 165T, was isolated from a dry land soil sample from the Gulbarga region, Karnataka province, India. The isolate produced yellow substrate mycelia and gray aerial mycelia on most tested media. Strain DAS 165T showed growth in the presence of 5 to 7% NaCl and at 45°C. The DNA G + C content was 69.7%. 16S rRNA gene sequence analysis together with these characteristics consistently assigned strain DAS 165T to the genus Streptomyces. The 16S rRNA gene sequence analysis revealed that strain DAS 165T was most closely related to S. tendae ATCC 19812T (D 63873) with a sequence similarity of 99.6% (three nucleotide differences out of 1,517). Strain DAS 165T formed a distinct clade based on analysis of the almost complete sequence and 120-nucleotide variable γ region of the 16S rRNA gene. Despite the high sequence similarity, strain DAS 165T was phenotypically different from S. tendae ATCC 19812T. DNA–DNA hybridization between these strains was 47% showing that strain DAS 165T is a distinct genomic species. Phenetic and genetic results support the classification of strain DAS 165T as a new species, for which the name S. tritolerans is proposed, with strain DAS 165T as the type strain (=DSM 41899T = CCTCCAA 206013T).

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Acknowledgments

This work was supported by the 21C Frontier Microbial Genomics and Application Centre program. Ministry of Science and Technology, Republic of Korea. And Wen-Jun Li was supported by Program for New Century Excellent Talent in University

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Authors and Affiliations

  1. Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 305-333, South Korea

    Dastager G. Syed, Chang-Jin Kim, Jae-Chan Lee & Dong-Jin Park

  2. The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650 091, P.R. China

    Wen-Jun Li, Li-Hua Xu, Xin-Peng Tian & Cheng-Lin Jiang

  3. Department of Studies and Research in Microbiology, Gulbarga University, Gulbarga, 585 106, India

    Dastager G. Syed & Dayanand Agasar

  4. Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, P.R. China

    Xin-Peng Tian

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  1. Dastager G. Syed
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  2. Dayanand Agasar
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  3. Chang-Jin Kim
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  4. Wen-Jun Li
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  5. Jae-Chan Lee
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  7. Li-Hua Xu
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  9. Cheng-Lin Jiang
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Correspondence to Dayanand Agasar.

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Syed, D.G., Agasar, D., Kim, CJ. et al. Streptomyces tritolerans sp. nov., a novel actinomycete isolated from soil in Karnataka, India. Antonie van Leeuwenhoek 92, 391–397 (2007). https://doi.org/10.1007/s10482-007-9166-2

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  • DOI: https://doi.org/10.1007/s10482-007-9166-2

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