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Molecular characterization of the full-length 23S and 5S ribosomal RNA (rRNA) genes of Taylorella asinigenitalis

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Abstract

An approximately 4.2 kbp region encoding 23S and 5S rRNA genes was identified when recombinant plasmid DNAs from two genomic DNA libraries and an inverse PCR product of Taylorella asinigenitalis UK-1 isolate were analyzed. Full-length genes of 23S rRNA (3,225 bp) and 5S rRNA (117 bp) of T. asinigenitalis are described. The present sequence analysis identified a non-coding hypothetically intrinsic transcription terminator region downstream of the 5S rRNA gene. The sequence, however, downstream of the 5S rRNA gene did not show any distal tRNA genes. Surprisingly, an intervening sequence (IVS) of 270 bp in length, including two specific tandem repeat units of 80 bp and one partial unit of 48 bp with unknown functions was identified in the first quarter of the 23S rRNA gene sequence. A second IVS of 70 bp in length was also identified in the central region of the 23S rRNA gene. In addition, by using PCR and sequencing procedures, two T. asinigenitalis isolates, UK-1 and UK-2, carried multiple IVSs in the first quarter and central regions. Moreover, the 23S rRNA fragmentation occurred in the UK-1 isolate. A phylogenetic analysis was first carried out based on the 23S rRNA sequence data from T. asinigenitalis UK-1 and 13 other β-Proteobacteria. This is the first report of IVSs in the 23S rRNA gene from the β-Proteobacteria.

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Abbreviations

bp:

Base pair

CEM:

Contagious equine metritis

IVS:

Intervening sequence

I:

Inverse

NJ:

Neighbour joining

np:

Nucleotide position

rRNA:

Ribosomal RNA

T :

Taylorella

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Authors and Affiliations

  1. Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Fuchinobe 1-17-71, Sagamihara, 229-8501, Japan

    Akihiro Tazumi, Satoru Saito, Tsuyoshi Sekizuka, Ohoshi Murayama & Motoo Matsuda

  2. Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, Bunkyoku Hongo 2-1-1, Tokyo, 113-8421, Japan

    Shinzaburo Takamiya

  3. Department of Bacteriology, Northern Ireland Public Health Laboratory, Belfast City Hospital, Belfast, BT9 7AD, Northern Ireland, UK

    John E. Moore & B. Cherie Millar

Authors
  1. Akihiro Tazumi
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  2. Satoru Saito
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  3. Tsuyoshi Sekizuka
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  4. Ohoshi Murayama
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  5. Shinzaburo Takamiya
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  6. John E. Moore
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  7. B. Cherie Millar
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  8. Motoo Matsuda
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Correspondence to Motoo Matsuda.

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Tazumi, A., Saito, S., Sekizuka, T. et al. Molecular characterization of the full-length 23S and 5S ribosomal RNA (rRNA) genes of Taylorella asinigenitalis . Antonie van Leeuwenhoek 92, 257–264 (2007). https://doi.org/10.1007/s10482-007-9144-8

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