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Purification and characterization of polygalacturonase produced by thermophilic Thermoascus aurantiacus CBMAI-756 in submerged fermentation

Antonie van Leeuwenhoek volume 91, pages 291–299 (2007)Cite this article

An Erratum to this article was published on 10 March 2007

Abstract

An extracellular polygalacturonase was isolated from 5-day culture filtrates of Thermoascus aurantiacus CBMAI-756 and purified by gel filtration and ion-exchange chromatography. The enzyme was maximally active at pH 5.5 and 60–65°C. The apparent K m with citrus pectin was 1.46 mg/ml and the V max was 2433.3 μmol/min/mg. The apparent molecular weight of the enzyme was 30 kDa. The enzyme was 100% stable at 50°C for 1 h and showed a half-life of 10 min at 60°C. Polygalacturonase was stable at pH 5.0–5.5 and maintained 33% of initial activity at pH 9.0. Metal ions, such as Zn+2, Mn+2, and Hg+2, inhibited 50, 75 and 100% of enzyme activity. The purified polygalacturonase was shown to be an endo/exo-enzyme, releasing mono, di and tri-galacturonic acids within 10 min of hydrolysis.

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Acknowledgments

The authors wish to thank the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for financial support.

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Authors and Affiliations

  1. Biologia, Universidade Estadual paulista-UNESP, Instituto de Biociências, Letras e Ciências Exatas-IBILCE, R: Crsitovao Colombo, 2265, Jardim Nazareth, São José do Rio Petro, São Paulo, 15054-000, Brazil

    Eduardo Silva Martins, Denis Silva, Rodrigo S. R. Leite & Eleni Gomes

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  1. Eduardo Silva Martins
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  2. Denis Silva
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  3. Rodrigo S. R. Leite
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  4. Eleni Gomes
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Correspondence to Eleni Gomes.

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An erratum to this article is available at http://dx.doi.org/10.1007/s10482-007-9162-6.

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Martins, E.S., Silva, D., Leite, R.S.R. et al. Purification and characterization of polygalacturonase produced by thermophilic Thermoascus aurantiacus CBMAI-756 in submerged fermentation. Antonie van Leeuwenhoek 91, 291–299 (2007). https://doi.org/10.1007/s10482-006-9114-6

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Keywords

  • Polygalacturonase
  • Purification
  • Thermoascus aurantiacus