Abstract
Interest in species of the genus Penicillium is related to their ability to produce the mycotoxin patulin and to cause spoilage of fruit products worldwide. The sequence of the isoepoxydon dehydrogenase (idh) gene, a gene in the patulin biosynthetic pathway, was determined for 28 strains representing 12 different Penicillium species known to produce the mycotoxin patulin. Isolates of Penicillium carneum, Penicillium clavigerum, Penicillium concentricum, Penicillium coprobium, Penicillium dipodomyicola, Penicillium expansum, Penicillium gladioli, Penicillium glandicola, Penicillium griseofulvum, Penicillium paneum, Penicillium sclerotigenum and Penicillium vulpinum were compared. Primer pairs for DNA amplification and sequencing were designed from the P. griseofulvum idh gene (GenBank AF006680). The two introns present were removed from the nucleotide sequences, which were translated to produce the IDH sequences of the 12 species for comparison. Phylogenetic relationships among the species were determined from rDNA (ITS1, 5.8 S, ITS2 and partial sequence of 28S rDNA) and from the idh nucleotide sequences minus the two introns. Maximum parsimony analysis showed trees based on rDNA and idh sequences to be congruent. It is anticipated that the genetic information obtained in the present study will aid in the design of probes, specific for patulin biosynthetic pathway genes, to identify the presence of these mycotoxigenic fungi.
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Acknowledgements
Special thanks are given to Amy E. Engberg for excellent technical assistance and to Dr. Cletus P. Kurtzman for generation of phylogenetic trees. Names of equipment and chemical supplies are necessary to report factually on experimental methods; however, the USDA neither guarantees nor warrants the standard of the products, and the use of the name by USDA implies no approval of the products to the exclusion of others that may also be suitable.
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Dombrink-Kurtzman, M.A. The sequence of the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway in Penicillium species. Antonie van Leeuwenhoek 91, 179–189 (2007). https://doi.org/10.1007/s10482-006-9109-3
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DOI: https://doi.org/10.1007/s10482-006-9109-3