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Cellular Lipid Fatty Acid Pattern Heterogeneity Between Reference and Recent Food Isolates of Listeria Monocytogenes as a Response to Cold Stress

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Abstract

Cells of four reference strains (Scott A, LO 28, CNL 895807 and ATCC 19115) and of five recent food isolates (A00M011, A00M018, A00M087, A00M092 and A00M123) of Listeria monocytogenes were grown until late exponential phase in Brain Heart Broth at two different temperatures (37 °C and 4 °C). Our results show that significant differences exist between the cellular lipid fatty acid profile of reference and recent food isolates. Like the reference strains, and in keeping with previous reports on the cellular lipid fatty acid profile of L. monocytogenes, the recent food isolates were characterised by the presence of ai15:0, i15:0 and ai17:0. In addition, the fatty acid ai13:0 was observed in all of the recent food isolates grown at 4 °C, whereas only two reference strains, Scott A and LO 28, showed ai13:0 in their cellular lipid fatty acid profile at 4 °C. When grown at 4 °C, the recent food isolates showed a mean aiC15/aiC17 ratio of 66, while reference strains were characterised by significantly lower ratios, ranging between 4.3 (ATCC 19115) and 28.9 (Scott A). These results showed that all of the recent food isolates, Scott A and LO28 strains use chain length and anteiso-branching (ai15:0) as their major response to cold temperature adaptation. However, the cold adaptation response of reference strains CNL 895807 and ATCC 19115 appears to be different.

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Authors and Affiliations

  1. Ecole Nationale Vétérinaire de Nantes – Route de Gachet, UMR-INRA SECALIM 1014, BP 40706, 44307, Nantes Cedex 03, France

    Margarida Ribeiro Neunlist, Michel Federighi & Michel Laroche

  2. Association pour le développement de la Recherche en Industries Agro-alimentaires (ADRIA), Unité Sécurité et Conservation des Aliments, Z.A. de Créac’h Gwen, 29196, Quimper Cedex, France

    Danièle Sohier

  3. LGPTA/INRA, 369 rue Jules Guesde , BP 39, F-59651, Villeneuve d’Ascq, France

    Gilles Delattre

  4. Laboratoire des Listeria, Centre National de référence des Listeria, Institut Pasteur, 25/28 rue du Docteur Roux, 75724, Paris, cedex 15, France

    Christine Jacquet

  5. Laboratoire de Microbiologie, Université des Sciences et Technologies de Lille, Bâtiment SN2, F-59655, Villeneuve d’Ascq Cedex, France

    Nour-Eddine Chihib

Authors
  1. Margarida Ribeiro Neunlist
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  2. Michel Federighi
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  3. Michel Laroche
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  4. Danièle Sohier
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  5. Gilles Delattre
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  6. Christine Jacquet
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  7. Nour-Eddine Chihib
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Correspondence to Nour-Eddine Chihib.

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Neunlist, M.R., Federighi, M., Laroche, M. et al. Cellular Lipid Fatty Acid Pattern Heterogeneity Between Reference and Recent Food Isolates of Listeria Monocytogenes as a Response to Cold Stress. Antonie Van Leeuwenhoek 88, 199–206 (2005). https://doi.org/10.1007/s10482-005-5412-7

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  • DOI: https://doi.org/10.1007/s10482-005-5412-7

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