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Yeast population dynamics of industrial fuel-ethanol fermentation process assessed by PCR-fingerprinting

Antonie van Leeuwenhoek volume 88, pages 13–23 (2005)Cite this article

An Erratum to this article was published on 01 August 2005

Abstract

Yeast population used in industrial production of fuel-ethanol may vary according to the plant process condition and to the environmental stresses imposed to yeast cells. Therefore, yeast strains isolated from a particular industrial process may be adapted to such conditions and should be used as starter strain instead of less adapted commercial strains. This work reports the use of PCR-fingerprinting method based on microsatellite primer (GTG)5 to characterize the yeast population dynamics along the fermentation period in six distilleries. The results show that indigenous fermenting strains present in the crude substrate can be more adapted to the industrial process than commercial strains. We also identified new strains that dominate the yeast population and were more present either in molasses or sugar cane fermenting distilleries. Those strains were proposed to be used as starters in those industrial processes. This is the first report on the use of molecular markers to discriminate Saccharomyces cerevisiae strains from fuel-ethanol producing process.

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Author notes

  1. Eurípedes Alves da Silva-Filho

    Present address: Departamento de Biologia, Universidade Federal de Alagoas, Brazil

Affiliations

  1. Setor de Biologia Molecular-LIKA, Brazil

    Eurípedes Alves da Silva-Filho, Marcos Antonio de Morais Jr & Diogo Ardaillon Simões

  2. Departamento de Genética, Universidade Federal de Pernambuco, Av. Moraes Rego, s/n Cidade Universitária, 50670-901, Recife, PE, Brazil

    Scheila Karina Brito dos Santos, Alecsandra do Monte Resende & Marcos Antonio de Morais Jr

  3. Departamento de Antibióticos, Universidade Federal de Pernambuco, Brazil

    José Otamar Falcão de Morais

  4. Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, PE, Brazil

    Diogo Ardaillon Simões

Authors
  1. Eurípedes Alves da Silva-Filho
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  2. Scheila Karina Brito dos Santos
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  3. Alecsandra do Monte Resende
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  4. José Otamar Falcão de Morais
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  5. Marcos Antonio de Morais Jr
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  6. Diogo Ardaillon Simões
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Corresponding author

Correspondence to Marcos Antonio de Morais Jr.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s10482-005-0901-2.

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Silva-Filho, E.A.d., Santos, S.K.B.d., Resende, A.d.M. et al. Yeast population dynamics of industrial fuel-ethanol fermentation process assessed by PCR-fingerprinting. Antonie Van Leeuwenhoek 88, 13–23 (2005). https://doi.org/10.1007/s10482-004-7283-8

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  • DOI: https://doi.org/10.1007/s10482-004-7283-8

Keywords

  • Ethanol fermentation
  • Industrial yeast adaptation
  • Microsatellite primer
  • PCR fingerprinting
  • Strain selection
  • Yeast succession