Impact of tree species on barley rhizosphere-associated fungi in an agroforestry ecosystem as revealed by 18S rDNA PCR-DGGE
Agroforestry systems have been considered a form of sustainable land use. Woody species in agroforestry systems can improve soil physicochemical properties by supplying leaf or stem litter. However, little is known about fungal community structure and diversity in agroforestry systems. In the present study, the culture-independent 18S rDNA-based polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method was used to investigate fungal community structure in rhizosphere and bulk soil in Populus euramevicana-barley and Taxodium distichum-barley agroforestry systems. DGGE profiling and cluster analysis revealed that the fungal community structure in the rhizosphere was more complex than that of bulk soil. Our results also indicated that the rhizosphere fungal community in barley was less affected by T. distichum than by P. euramevicana. In addition, an increase in the relative abundance of certain rhizosphere fungal populations was detected in this agroforestry system. Sequencing of prominent DGGE bands revealed an increase in the rhizosphere of a fungal species belonging to the genera Chaetomium, which includes potential biocontrol agents. A rare cellulolytic fungus, Acremonium alcalophilum, was found in the bulk soil from P. euramevicana and barley grown under P. euramevicana. Taken together, our findings may provide new insights into agroforestry practices.
KeywordsPCR-DGGE Fungal community Rhizosphere Agroforestry
This study was funded by the National Forestry Public Welfare Industry Research Project (201304211), the Collaborative Innovation Plan of Jiangsu Higher Education, the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and Central Government Funding of Forestry Science and Technology Demonstration Projects (TJS1). The authors are grateful to the anonymous reviewers for their valuable suggestions.
Compliance with ethical standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- Fanish SA, Priya RS (2013) Review on benefits of agroforestry system. Int J Edu Res 1:1–12Google Scholar
- McNear DH Jr (2013) The rhizosphere-roots, soil and everything in between. Nat Educ Knowl 4(3):1Google Scholar
- O'Callaghan M, Lorenz N, Gerard EM (2006) Characterization of phylloplane and rhizosphere microbial populations using PCR and denaturing gradient gel electrophoresis (DGGE). In: Cooper JE, Rao JR (eds) Molecular approaches to soil, rhizosphere and plant microorganism analysis. CABI international, Oxfordshire, UK, pp 99–115CrossRefGoogle Scholar
- Smalla K, Wieland G, Buchner A, Zock A, Parzy J, Kaiser S, Roskot N, Heuer H, Berg G (2011) Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: plant-dependent enrichment and seasonal shifts revealed. Appl Environ Microbiol 67:4742–4751CrossRefGoogle Scholar
- Tangjang S, Arunachalam K, Arunachalam A, Shukla AK (2009) Microbial population dynamics of soil under traditional agroforestry systems in northeast India. Res J Soil Biol 1:1–7Google Scholar
- Thorn G (1997) The fungi in soil. In: van Elsas JD, Trevors JT, Wellington EMH (eds) Modern soil microbiology. Marcel Dekker Inc, New York, pp 63–127Google Scholar
- Uren NC (2000) Types, amounts and possible functions of compounds released into the rhizosphere by soil grown plants. In: Pinton R, Varani Z, Nanniperi P (eds) The rhizosphere: biochemistry, and organic substances at the soil interface. Marcel Dekker Inc., New York, pp 19–40Google Scholar