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Potential impact of prickly pear cactus flour and Salix babylonica extract on cecal fermentation and methane production in horses

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Abstract

The cecal gas (GP) and methane (CH4) production and cecal fermentation kinetics when corn grain (CG) was replaced with prickly cactus (PC) in a horse’s diet at different levels of Salix babylonica (SB) extract was investigated. Three total mixed rations where CG was replaced with PC at three levels (/kg): 0 g (Control), 75 g (PC75) or 150 g (PC150) were prepared and SB extract added at four levels: 0, 0.6, 1.2 and 1.8 mL/g dry matter (DM) of substrates. No ration type × SB extract dose interaction was observed (P > 0.05) for GP kinetics and CH4 production. Increasing the level of PC in the ration quadratically increased (P < 0.01) the asymptotic GP and decreased (P < 0.01) the rate and lag time of GP. Increasing the level of PC in the ration, increased GP values (P < 0.05). Increasing the level of SB extract linearly decreased (P = 0.001) the lag time of GP of all diets without affecting the asymptotic GP or the rate of GP. Ration type and SB level had no effect (P > 0.05) on CH4 production; however, at 36 h of incubation, SB extract decreased CH4 production. The rations PC75 and PC150 increased cecal pH compared with the control ration. The PC150 ration had the highest (P < 0.05) DM degradability, short chain fatty acids production, and gas yield after 24 h of incubation, with no effect (P > 0.05) of SB inclusion on all investigated fermentation kinetic parameters. It is concluded that increasing the level of PC in the diet of horse and replacing CG up to 60%, increased GP and improved cecal fermentation kinetics without affecting CH4 production. Inclusion of S. babylonica extract in the tested rations had weak effects on fermentation kinetics although it decreased the lag time of GP.

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References

  • Ahmed MH, Elghandour MMY, Salem AZM, Zeweil HS, Kholif AE, Klieve AV, Abdelrassol AMA (2015) Influence of Trichoderma reesei or Saccharomyces cerevisiae on performance, ruminal fermentation, carcass characteristics and blood biochemistry of lambs fed Atriplex nummularia and Acacia saligna mixture. Livest Sci 180:90–97

    Article  Google Scholar 

  • AOAC (1997) Association of official analytical chemists. Official methods of analysis, 16th edn. AOAC, Arlington

  • Blümmel M, Ørskov ER (1993) Comparison of in vitro gas production and nylon bag degradability of roughages in predicting feed intake in cattle. Anim Feed Sci Technol 40:109–119

    Article  Google Scholar 

  • Blümmel M, Steingss H, Becker K (1997) The relationship between in vitro gas production, in vitro microbial biomass yield and 15N incorporation and its implications for the prediction of voluntary feed intake of roughages. Br J Nutr 77:911–921

    Article  PubMed  Google Scholar 

  • Cedillo J, Vázquez-Armijo JF, González-Reyna A, Salem AZM, Kholif AE, Hernández-Meléndez J, Martínez-González JC, de Oca Jiménez RM, Rivero N, López D (2014) Effects of different doses of Salix babylonica extract on growth performance and diet in vitro gas production in Pelibuey growing lambs. Ital J Anim Sci 13(3):609–613

    Article  Google Scholar 

  • Cedillo J, Kholif AE, Salem AZM, Elghandour MMY, Vázquez JF, Alonso MU, Barbabosa A, Chagoyán JCV, Reyna AG (2015) Oral administration of Sauce llorón extract to growing lambs to control gastrointestinal nematodes and Moniezia spp. Asian Pac J Trop Med 8(7):520–525

    Article  PubMed  CAS  Google Scholar 

  • Costa RG, Filho EMB, de Medeiros AN, Givisiez PEN, Queirogac RCRE, Silva Melo AAS (2009) Effects of increasing levels of cactus pear (Opuntia ficus-indica L. Miller) in the diet of dairy goats and its contribution as a source of water. Small Rumin Res 82:62–65

    Article  Google Scholar 

  • Dhanoa MS, Lopez S, Dijkstra J (2000) Estimating the extent of degradation of ruminant feeds from a description of their gas production profiles observed in vitro: comparison of models. Br J Nutr 83:131–142

    Article  PubMed  CAS  Google Scholar 

  • Elghandour MMY, Salem AZM, Gonzalez-Ronquillo M, Bórqueza JL, Gado HM, Odongo NE, Penuelas CG (2013) Effects of exogenous enzymes on in vitro gas production kinetics and ruminal fermentation of four fibrous feeds. Anim Feed Sci Technol 179:46–53

    Article  CAS  Google Scholar 

  • Elghandour MMY, Kholif AE, Bastida AZ, Martínez DLP, Salem AZM (2015a) In vitro gas production of five rations of different maize silage and concentrate ratios influenced by increasing levels of chemically characterized extract of Salix babylonica. Turk J Vet Anim Sci 39(2):186–194

    Article  CAS  Google Scholar 

  • Elghandour MMY, Kholif AE, Marquez-Molina O, Vazquez-Armijo JF, Puniya AK, Salem AZM (2015b) Influence of individual or mixed cellulase and xylanase mixture on in vitro rumen gas production kinetics of total mixed rations with different maize silage and concentrate ratios. Turk J Vet Anim Sci 39(4):435–442

    Article  CAS  Google Scholar 

  • Elghandour MMY, Kholif AE, Hernández J, Mariezcurrena MD, López S, Camacho LM, Márquez O, Salem AZM (2016a) Influence of the addition of exogenous xylanase with or without pre-incubation on the in vitro ruminal fermentation of three fibrous feeds. Czech J Anim Sci 61(6):262–272

    Article  CAS  Google Scholar 

  • Elghandour MMY, Kholif AE, Salem AZM, de Oca RM, Barbabosa A, Mariezcurrena M, Olafadehan OA (2016b) Addressing sustainable ruminal methane and carbon dioxide emissions of soybean hulls by organic acid salts. J Clean Prod 135:194–200

    Article  CAS  Google Scholar 

  • Elghandour MMY, Kholif AE, Salem AZM, Olafadehan OA, Kholif AM (2016c) Sustainable anaerobic rumen methane and carbon dioxide productions from prickly pear cactus flour by organic acid salts addition. J Clean Prod 139:1362–1369

    Article  CAS  Google Scholar 

  • Elghandour MMY, Vázquez JC, Salem AZM, Kholif AE, Cipriano MM, Camacho LM, Márquez O (2016d) In vitro gas and methane production of two mixed rations influenced by three different cultures of Saccharomyces cerevisiae. J Appl Anim Res 45(1):389–395

    Article  CAS  Google Scholar 

  • Forsberg C, Forano E, Chesson A (2000) Microbial adherence to the plant cell wall and enzymatic hydrolysis. In: Cronje PB (ed) Ruminant physiology: digestion, metabolism, growth and reproduction. CABI Publishing, Wallingford, pp 79–97

    Chapter  Google Scholar 

  • France J, Dijkstra J, Dhanoa MS, López S, Bannink A (2000) Estimating the extent of degradation of ruminant feeds from a description of their gas production profiles observed in vitro: derivation of models and other mathematical considerations. Br J Nutr 83:143–150

    Article  PubMed  CAS  Google Scholar 

  • Getachew G, Makkar HPS, Becker K (2002) Tropical browses: contents of phenolic compounds, in vitro gas production and stoichiometric relationship between short chain fatty acid and in vitro gas production. J Agric Sci Camb 139:341–352

    Article  CAS  Google Scholar 

  • Goering MK, Van Soest PJ (1970) Forage fiber analysis (apparatus, reagents, procedures and some applications). Agriculture handbook, No. 379. Agricultural Research Service, USDA, Washington, DC

  • Hart KJ, Yanez-Ruiz DR, Duval SM, McEwan NR, Newbold CJ (2008) Plant extracts to manipulate rumen fermentation. Anim Feed Sci Technol 147:8–35

    Article  CAS  Google Scholar 

  • Intergovernmental Panel on Climate Change (2008) Climate change, 2007. Synthesis report. Contribution of working group I, II, and III to the fourth assessment report of the intergovernmental panel on climate change. IPCC, Geneva

  • Johnson KA, Johnson DE (1995) Methane emissions from cattle. J Anim Sci 73:2483–2492

    Article  PubMed  CAS  Google Scholar 

  • Khazaal K, Dentinho MT, Ribeiro JM, Ørskov ER (1995) Prediction of apparent digestibility and voluntary feed intake of hays fed to sheep: comparison between using fibre component, in vitro digestibility or characteristics of gas production or nylon bag degradation. Anim Sci 61:521–538

    Article  Google Scholar 

  • Kholif AE, Gouda GA, Morsy TA, Salem AZM, Lopez S, Kholif AM (2015) Moringa oleifera leaf meal as a protein source in lactating goat’s diets: feed intake, digestibility, ruminal fermentation, milk yield and composition, and its fatty acids profile. Small Rumin Res 129:129–137

    Article  Google Scholar 

  • Kholif AE, Baza-García LA, Elghandour MMY, Salem AZM, Barbabosa A, Dominguez-Vara IA, Sanchez-Torres JE (2016a) In vitro assessment of fecal inocula from horses fed on high-fiber diets with fibrolytic enzymes addition on gas, methane, and carbon dioxide productions as indicators of hindgut activity. J Equine Vet Sci 39:44–50

    Article  Google Scholar 

  • Kholif AE, Morsy TA, Abd El Tawab AM, Anele UY, Galyean ML (2016b) Effect of supplementing diets of Anglo-Nubian goats with soybean and flaxseed oils on lactational performance. J Agric Food Chem 64(31):6163–6170

    Article  PubMed  CAS  Google Scholar 

  • Menke KH, Raab L, Salewski A, Steingass H, Fritz D, Schneider W (1979) The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro. J Agric Sci Camb 93:217–222

    Article  CAS  Google Scholar 

  • Morsy TA, Kholif AE, Kholif SM, Kholif AM, Sun X, Salem AZM (2016) Effects of two enzyme feed additives on digestion and milk production in lactating Egyptian buffaloes. Ann Anim Sci 16:209–222

    Article  CAS  Google Scholar 

  • Nsereko VL, Beauchemin KA, Morgavi DP, Rode LM, Furtado AF, McAllister TA, Iwaasa AD, Yang WZ, Wang Y (2002) Effect of a fibrolytic enzyme preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows. Can J Microbiol 48:14–20

    Article  PubMed  CAS  Google Scholar 

  • Okunade SA, Olafadehan OA, Isah OA (2014) Fodder potential and acceptability of selected tree leaves by goats. Anim Nutr Feed Technol 14:489–498

    Article  Google Scholar 

  • Paya H, Taghizadeh A, Janmohammadi H, Moghadam GA (2007) Nutrient digestibility and gas production of some tropical feeds used in ruminant diets estimated by the in vivo and in vitro gas production techniques. Am J Anim Vet Sci 2:108–113

    Article  Google Scholar 

  • Rivero N, Salem AZM, Ayala M, Elghandour MMY, Kholif AE, Barbabosa A, Camacho LM, Rojas S, Olivares J, Cipriano M (2016) Influence of Salix babylonica extract, exogenous enzyme of xylanase and their combination on blood hematological and biochemical profile in sheep and goats. Indian J Anim Sci 86(10):1140–1144

    CAS  Google Scholar 

  • Rodriguez MP, Mariezcurrena MD, Mariezcurrena MA, Lagunas BC, Elghandour MMY, Kholif AM, Kholif AE, Almaráz EM, Salem AZM (2015) Influence of live cells or cells extract of Saccharomyces cerevisiae on in vitro gas production of a total mixed ration. Ital J Anim Sci 14:590–595

    Article  Google Scholar 

  • Salem AZM, Kholif AE, Elghandour MMY, Buendía G, Mariezcurrena MD, Hernandez SR, Camacho LM (2014a) Influence of oral administration of Salix babylonica extract on milk production and composition in dairy cows. Ital J Anim Sci 13(1):10–14

    Article  Google Scholar 

  • Salem AZM, Ryena AC, Elghandour MMY, Camacho LM, Kholif AE, Salazar MC, Domínguez IA, Jiménez RM, Almaraz EM, Martínez AGL, Mariezcurrena MA (2014b) Influence of Salix babylonica extract in combination or not with increasing levels of minerals mixture on in vitro rumen gas production kinetics of a total mixed ration. Ital J Anim Sci 13:873–879

    Google Scholar 

  • Salem AZ, Elghandour MM, Kholif AE, Barbabosa A, Camacho LM, Odongo NE (2016a) Influence of feeding horses a high fiber diet with or without live yeast cultures supplementation on feed intake, nutrient digestion, blood chemistry, fecal coliform count, and in vitro fecal fermentation. J Equine Vet Sci 39:12–19

    Article  Google Scholar 

  • Salem AZM, Elghandour MMY, Kholif AE, López S, Pliego AB, Cipriano-Salazar M, Chagoyán JCV, de Oca Jiménez RM, Alonso MU (2016b) Tree leaves of Salix babylonica extract as a natural anthelmintic for small-ruminant farms in a semiarid region in Mexico. Agrofor Syst. doi:10.1007/s10457-016-9909-z

    Article  Google Scholar 

  • Salem AZM, Kholif AE, Elghandour MMY, Hernández J, Limas A, Cipriano M, Camacho L, Rojas S, Olivares J (2016c) Influence of Salix babylonica extract addition on in vitro rumen gas production and degradability of ryegrass silage harvested in different cutting days. Indian J Anim Sci 86(9):1030–1035

    Google Scholar 

  • SAS (2002) Statistical analysis system. User’s guide: statistics. Ver 9.0. SAS Institute, Cary

  • Stintzing G, Carle R (2005) Cactus stems (Opuntia spp.): a review on their chemistry and uses. Mol Nutr Food Res 49:175

    Article  PubMed  CAS  Google Scholar 

  • Theodorou MK, Williams BA, Dhanoa MS, McAllan AB, France J (1994) A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim Feed Sci Technol 48:185–197

    Article  Google Scholar 

  • Valdes KI, Salem AZM, López S, Alonso MU, Rivero N, Elghandour MMY, Domínguez IA, Ronquillo MG, Kholif AE (2015) Influence of exogenous enzymes in presence of Salix babylonica extract on digestibility, microbial protein synthesis and performance of lambs fed maize silage. J Agric Sci Camb 153:732–742

    Article  CAS  Google Scholar 

  • Vallejo L, Salem AZM, Kholif AE, Elghangour M, Fajardo R, Rivero N, Bastida A, Mariezcurrena M (2016) Influence of cellulase or xylanase on the in vitro rumen gas production and fermentation of corn stover. Indian J Anim Sci 86(1):70–74

    Google Scholar 

  • Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fibre, neutral detergent fibre, and non-starch carbohydrates in relation to animal nutrition. J Dairy Sci 74:3583–3597

    Article  PubMed  Google Scholar 

  • Wanderley WL, Ferreira MA, Andrade DKB, Véras ASC, Farias I, Lima LE, Dias AMA (2002) Replacement of forage cactus (Opuntia ficus indica Mill) for sorghum silage (Sorghum bicolor (L.) Moench) in the dairy cows feeding. Rev Bras Zootec 31:273–281

    Article  Google Scholar 

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Acknowledgements

Authors would like to thank the Universidad Autónoma del Estado de México (project # UAEM 4120/2016SF).

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Authors and Affiliations

  1. Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca, Mexico

    A. Parra-Garcia, A. Z. M. Salem & M. M. Y. Elghandour

  2. Unidad Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Guerrero, Cd. Altamirano-Iguala, Chilpancingo, Guerrero, Mexico

    L. M. Camacho

  3. Department of Animal Sciences, School of Agriculture, Pwani University, P. O. Box 195-80108, Kilifi, Kenya

    N. E. Odongo

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  1. A. Parra-Garcia
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  2. A. Z. M. Salem
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  4. L. M. Camacho
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Correspondence to A. Z. M. Salem.

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Parra-Garcia, A., Salem, A.Z.M., Elghandour, M.M.Y. et al. Potential impact of prickly pear cactus flour and Salix babylonica extract on cecal fermentation and methane production in horses. Agroforest Syst 92, 1145–1154 (2018). https://doi.org/10.1007/s10457-016-0051-8

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