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Agroforestry Systems

, Volume 71, Issue 3, pp 195–200 | Cite as

Rapid clonal multiplication of a woody tree, Vitex negundo L. through axillary shoots proliferation

  • Naseem Ahmad
  • Mohammad AnisEmail author
Article

Abstract

An efficient and improved shoot regeneration technique for the micropropagation of Vitex negundo, an aromatic and medicinal shrub through in vitro culture of nodal segments with axillary buds, is described. Thidiazuron (TDZ) used at 1.0 μM was the most effective in inducing bud break and growth, and also in initiating multiple shoot proliferation at the rate of 25 microshoots per nodal explant with axillary buds, after 4 weeks of culture. By repeated subculturing of nodal explants, a high-frequency multiplication rate was established. Optimum shoot multiplication and elongation was achieved when TDZ exposed explants were subcultured on Murashige and Skoog (MS) media containing a combination of 1.0 μM 6-benzyladenine (BA) and 0.5 μM α-naphthalene acetic acid (NAA). Efficient rooting was achieved directly in soilrite when basal portion of the shoots were treated with 500 μM indole-3-butyric acid for 10 min which was the most effective in inducing roots, as 97% of the microshoots produced roots. Plantlets went through a hardening phase in a controlled plant growth chamber, prior to ex-vitro transfer. Micropropagated plants grew well, attained maturity and flowered. No phenotypical differences for morphogenesis were observed among the regenerants.

Keywords

Axillary shoot multiplication Nodal segment Plant regeneration Thidiazuron 

Abbreviation

BA

6-Benzyladenine

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

Kinetin

6-Furfurylaminopurine

MS

Murashige and Skoog medium

NAA

α-Naphthalene acetic acid

TDZ

Thidiazuron

Notes

Acknowledgements

The award of a Senior Research Fellowship to N A by the Council of Scientific and Industrial Research (CSIR), Government of India, New Delhi, is greatly acknowledged. Research support from The Department of Science and Technology (Govt. of India) New Delhi under the FIST-DST Programme, is also acknowledged.

References

  1. Ahmad N, Siddiqui I, Anis M (2006) Improved plant regeneration in chilli using thidiazuron. Biol Plant 50:701–704CrossRefGoogle Scholar
  2. Anonymous (2003) The wealth of India, raw materials, Vol. I. Publication and Information Directorate, CSIR, New Delhi, India, pp. 522–524Google Scholar
  3. Chandramu C, Rao MD, Reddy VD (2003) High frequency induction of multiple shoots from nodal explants of Vitex negundo L. using sodium sulphate. J Plant Biotec 5:107–113Google Scholar
  4. Faisal M, Ahmad N, Anis M (2005) Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tiss Org Cult 80:187–190CrossRefGoogle Scholar
  5. Faisal M, Ahmad N, Anis M (2005)In vitro regeneration and mass propagation of Ruta graveolens L. A multiple shrub. HortScience 40:1487–1480Google Scholar
  6. Huettman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss Org Cult 33:105–119CrossRefGoogle Scholar
  7. Khurana P, Bhatnagar S, Kumari S (2005) Thidiazuron and woody plant tissue culture. J Plant Biol 32:1–12Google Scholar
  8. Kim MK, Sommer HE, Bongarten BC, Markle SA (1997) High-frequency induction of adventitious shoots from hypocotyls segments of Liquidambar styraciflua L. by thidiazuron. Plant Cell Rep 16:536–540Google Scholar
  9. Kukreja AK, Mathur AK, Zaim M (1990) Mass production of virus free patchauli plants [Pogostemon cablin (Blanco) Benth]. Trop Agric 67:101–104Google Scholar
  10. Mok MC, Mok DWS, Armstrong DJ, Shudo K, Isogai Y, Okamoto T (1982) Cytokinin activity of N-phenyl-N’-1, 2, 3-thidiazol-5-ylurea (thidiazuron). Phytochemistry 21:1509–1511CrossRefGoogle Scholar
  11. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–497CrossRefGoogle Scholar
  12. Sahoo Y, Chand PK (1998) Micropropagation of Vitex negundo L., a woody aromatic medicinal shrub, through high-frequency axillary shoot proliferation. Plant Cell Rep 18:301–307CrossRefGoogle Scholar
  13. Sen J, Sharma AK (1991) Micropropagation of Withania somnifera from germinating seeds and shoot tips. Plant Cell Tiss Org Cult 26:71–73CrossRefGoogle Scholar
  14. Vadawale AV, Brave DM, Dave AM (2006) In vitro flowering and rapid propagation of Vitex negundo L. A medicinal plant. Indian J Biotec 5:112–116Google Scholar
  15. Wang SY, Steffens GL, Faust M (1986) Breaking bud dormancy in apple with a plant bio regulator, thidiazuron. Phytochemistry 25:311–317CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Plant Biotechnology Laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia

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