It is possible that current tree domestication practices undertaken by farmers reduce the genetic base of tree resources on farms, raising concerns regarding the productivity, sustainability and conservation value of agroforestry ecosystems. Here, we assessed possible changes in genetic variation during domestication in the important and heavily utilised timber species, Vitex fischeri Gürke (syn. Vitex keniensis), by comparing geographically proximate forest and farm material in central Kenya. Employing RAPD analysis, a total of 104 polymorphic markers revealed by five arbitrary primers were scored in a total of 65 individuals, 32 from forest and 33 from farmland. Despite concerns of possible genetic erosion, forest and farm stands did not differ significantly in levels of genetic variation, with H values of 0.278 and 0.269, respectively. However, Mantel tests did reveal greater geographically related associative genetic structure among individuals in farm rather than forest material, with r M values of 0.217 and 0.114, respectively. A more detailed analysis of structure suggested this could be due to local variation in origin of some on-farm trees. Implications of data for the genetic management of V. fischeri stands during farmer-led tree domestication activities are discussed. At present, there appears little reason to reject on-farm V. fischeri as a source of germplasm for future on-farm planting or for conservation purposes, although this situation may change and will require monitoring.
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Agufa C.A.C. 2002. Genetic variation in Sclerocarya birrea and Uapaca kirkiana – indigenous fruit trees of the Miombo woodlands, M.Sc. Thesis. Jomo Kenyatta University of Agriculture and Technology, NairobiKenya.
Ahenda J.O. 1999. Taxonomy and genetic structure of Meru oak populations, Vitex keniensis Turrill and Vitex fischeri Gürkein East AfricaPh.D. Thesis. Wageningen University, Wageningen, The Netherlands.
J.E.M. Arnold P.A. Dewees (Eds) (1995) Tree Management in Farmer Strategies: Responses to Agricultural Intensification Oxford University Press London, United Kingdom
F. Austerlitz S. Mariette N. Machon P.-H. Gouyon B. Godelle (2000) ArticleTitleEffects of colonization processes on genetic diversity: differences between annual plants and tree species Genetics 154 1309–1321 Occurrence Handle10757772 Occurrence Handle1:STN:280:DC%2BD3c3itFOgtw%3D%3D
Betser L.J., Mugwe J. and Muriuki J. 2000. On-farm production and marketing of high-value tree products in the central highlands of Kenya. In: Temu A.B., Lund G., Malimbwi R.E., Kowero G.S., Klein K. and Malende Y. (eds.) Off-forest tree resources of Africa. Proceedings of a workshop held in ArushaTanzania 1999. The African Academy of Sciences, Nairobi, Kenya, pp. 226–240.
W.C. Black SuffixIV (1993) ArticleTitlePCR with arbitrary primers: approach with care Insect Mol. Biol. 2 1–6 Occurrence Handle9087536 Occurrence Handle1:CAS:528:DyaK2cXhvVCjurk%3D
Boshier D.H. 2000. Mating systems. In: Young A., Boshier D. and Boyle T. (eds.) Forest Conservation Genetics: Principles and Practice. CSIRO Publishing, Melbourne, Australia and CABI Publishing, Wallingford, United Kingdom, pp. 63–79.
J.-M. Bouvet C. Fontaine H. Sanou C. Cardi (2004) ArticleTitleAn analysis of the pattern of genetic variation in Vitellaria paradoxa using RAPD markers Agroforest. Syst. 60 61–69 Occurrence Handle10.1023/B:AGFO.0000009405.74331.74
Brodie A.W., Labarta-Chavarri R.A. and Weber J.C. 1997. Tree Germplasm Management and Use On-farm in the Peruvian Amazon: A Case Study from the Ucayali Region, Peru. ODI, London, United Kingdom and ICRAF, Nairobi, Kenya.
J.R. Chamberlain (1998) ArticleTitleIsozyme variation in Calliandra calothyrsus (Leguminosae): its implications for species delimitation and conservation Am. J. Bot. 85 37–47 Occurrence Handle10.2307/2446552
I.K. Dawson A.J. Simons R. Waugh W. Powell (1995) ArticleTitleDiversity and genetic differentiation among subpopulations of Gliricidia sepium revealed by PCR-based assays Heredity 74 IssueID10 18
J.J. Doyle J.L. Doyle (1987) ArticleTitleCTAB DNA extraction in plants Phytochem. Bull. 19 IssueID11 15
O. Hammer D.A.T. Harper P.D. Ryan (2002) PAST – Paleontological Statistics. Version 0.82 Paleontological Museum, University of Oslo Oslo, Norway
C. Holding S. Carsan (2001) Proceedings of the Second Meru Timber Marketing Stakeholders Workshop. An activity of the Meru Timber Marketing Programme (FAN/MOARD/ICRAF) ICRAF Nairobi, Kenya
C. Holding W. Omondi (Eds) (1998) Evolution of Provision of Tree Seed in Extension Programmes: Case Studies from Kenya and Uganda Swedish International Development Cooperation Agency Nairobi, Kenya
P.M. Hollingsworth I.K. Dawson W.P. Goodall-Copestake J.E. Richardson J.C. Weber C. Sotelo Montes R.T. Pennington (2005) ArticleTitleDo farmers reduce genetic diversity when they domesticate tropical trees? A case study from Amazonia Mol. Ecol. 14 497–501 Occurrence Handle15660940 Occurrence Handle1:CAS:528:DC%2BD2MXit1alsrs%3D Occurrence Handle10.1111/j.1365-294X.2005.02431.x
R. Jamnadass J. Hanson J. Poole O. Hanotte A.J. Simons I.K. Dawson (2005) ArticleTitleHigh differentiation among populations of the woody legume Sesbania sesban in sub-Saharan Africa: implications for conservation and cultivation during germplasm introduction into agroforestry systems Forest Ecol. Manage. 210 225–238 Occurrence Handle10.1016/j.foreco.2005.02.033
E. Jenczewski J.M. Prosperi J. Ronfort (1999) ArticleTitleDifferentiation between natural and cultivated populations of Medicago sativa (Leguminosae) from Spain: analysis with random amplified polymorphic DNA (RAPD) markers and comparison to allozymes Mol. Ecol. 8 1317–1330 Occurrence Handle10447872 Occurrence Handle1:STN:280:DyaK1Mzns1Omsg%3D%3D Occurrence Handle10.1046/j.1365-294X.1999.00697.x
B.A. Kelly O.J. Hardy J.-M. Bouvet (2004) ArticleTitleTemporal and spatial genetic structure in Vitellaria paradoxa (shea tree) in an agroforestry system in southern Mali Mol. Ecol. 13 1231–1240 Occurrence Handle15078458 Occurrence Handle1:CAS:528:DC%2BD2cXktl2rsbo%3D Occurrence Handle10.1111/j.1365-294X.2004.02144.x
R. Kindt (1997) Local perceptions on tree propagation and domestication: results from a survey in Western Kenya ICRAF Nairobi Kenya
R. Kindt (2002) Methodology for Tree Species Diversification Planning in African Agroecosystems University of Gent Gent, Belgium
Kindt R. and Lengkeek A.G. 1999. Tree diversity on farm - use it or lose it. In: Agricultural Biodiversity Conservation. Proceedings of a workshop held in Nairobi, Kenya, January 1999. ITDG East Africa, Nairobi, Kenya, pp. 75–85.
KWS (1999) Aerial Survey of the Destruction of Mt. KenyaImenti and Ngare Ndare Forest Reserves Kenya Wildlife Service Nairobi, Kenya
P. Legendre L. Legendre (1998) Numerical Ecology Elsevier Science Amsterdam, The Netherlands
A.G. Lengkeek (2003) Diversity makes a difference: farmers managing inter- and intra- specific tree species diversity in Meru Kenya Wageningen University Wageningen, The Netherlands
A.G. Lengkeek S. Carsan (2004) ArticleTitleThe process of a participatory tree domestication project in MeruKenya Dev. Practice 14 445–451
Lengkeek A.G., Kindt R., van der Maesen L.J.G., Simons A.J. and van Oijen D.C.C. 2005a. Tree density and germplasm source in agroforestry ecosystems, a case study from Meru Mt Kenya. Genet. Resour. Crop Evol. 52(6): 709–721.
A.G. Lengkeek H. Jaenicke I.K. Dawson (2005b) ArticleTitleGenetic bottlenecks in agroforestry systems: results of tree nursery surveys in East Africa Agroforest. Syst. 63 149–155 Occurrence Handle10.1007/s10457-004-9155-7
A.J. Lowe A.C.M. Gillies J. Wilson I.K. Dawson (2000) ArticleTitleConservation genetics of bush mango from central/west Africa: implications from random amplified polymorphic DNA analysis Mol. Ecol. 9 831–841 Occurrence Handle10886647 Occurrence Handle1:STN:280:DC%2BD3M3pvFOhtQ%3D%3D Occurrence Handle10.1046/j.1365-294x.2000.00936.x
M. Lynch B.G. Milligan (1994) ArticleTitleAnalysis of population genetic structure with RAPD markers Mol. Ecol. 3 91–99 Occurrence Handle8019690 Occurrence Handle1:STN:280:ByuB1M%2Fms1E%3D
N. Mantel (1967) ArticleTitleThe detection of disease clustering and a generalized regression approach Cancer Res. 27 209–220 Occurrence Handle6018555 Occurrence Handle1:STN:280:CCiD1MzgsVY%3D
B. McCune R.J. Mefford (1999) PC-ORD for Windows. Multivariate Analysis of Ecological Data. Version 4.10 MjM Software Gleneden Beach, Oregon, USA
InstitutionalAuthorNameMOA (2000) Timber Marketing in Igoki and Ntakira Locations of Meru Central District Kenya Ministry of Agriculture and Rural Development Meru, Kenya
G.M. Muluvi J.I. Sprent N. Soranzo J. Provan D. Odee G. Folkard J.W. McNicol W. Powell (1999) ArticleTitleAmplified fragment length polymorphism (AFLP) analysis of genetic variation in Moringa oleifera Lam Mol. Ecol. 8 463–470 Occurrence Handle10199008 Occurrence Handle1:CAS:528:DyaK1MXjtVKht7Y%3D Occurrence Handle10.1046/j.1365-294X.1999.00589.x
A.M. Muchugi-Mwangi (2001) Genetic variation in the threatened medicinal tree Prunus africana (Hook F) in Kenya and Cameroon: implications for the genetic management of the species Kenyatta University Nairobi, Kenya
M. Nei (1978) ArticleTitleEstimation of average heterozygosity and genetic distance from a small number of individuals Genetics 89 583–590
InstitutionalAuthorNameSGRP (2000) Genetic resources management in ecosystems CIFOR Bogor, Indonesia
A.J. Simons (1996) Delivery of improvement for agroforestry trees M.J. Dieters A.C. Matheson D.G. Nikles C.E. Harwood S.M. Walker (Eds) Tree Improvement for Sustainable Tropical Forestry Queensland Forestry Research Institute Gympie, Australia 391–400
A.J. Simons D.J. MacQueen J.L. Stewart (1994) Strategic concepts in the domestication of non-industrial trees R.R.B. Leakey A.C. Newton (Eds) Tropical Trees: the Potential for Domestication and the Rebuilding of Forest Resources HMSO London, UK 91–102
Simons A.J., Jaenicke H., Tchoundjeu Z., Dawson I., Kindt R., Oginosako Z., Lengkeek A. and De Grande A. 2000. The future of trees is on farm: tree domestication in Africa. In: Krishnapillay B., Soepadmo E., Lotfy Arshad N., Wong A., Appanah S., Wan Chik S., Manokaran N., Lay Tong H. and Kean Choon K. (eds.) Forests and Society: the Role of Research. Proceedings of the XXIst IUFRO World Congress, held in Kuala Lumpur, Malaysia, August 2000. IUFRO Secretariat, Vienna, Austria and the Forest Research Institute Malaysia, Kuala Lumpur, Malaysia, Sub-plenary Sessions Vol. 1, pp. 752–760.
T. Sorensen (1948) ArticleTitleA method of establishing groups of equal amplitude in plant sociology based on similarity of species content and its application to analysis of the vegetation on Danish commons Biologiske Skrifter 5 1–34
Tapia M.E. and De la Torre A. 1998. Women Farmers and Andean Seeds. Gender and Genetic Resources Management. FAO, Rome, Italy and IPGRI, Rome, Italy.
D.C.C. Oijen Particlevan (2002) Will the Spirit of nGai Survive? A Tree Survey on Ethnobotany, Conservation Potential and Biomass on 35 farms in Meru, Kenya Wageningen University Wageningen, The Netherlands
J.C. Weber R.L. Labarta Chávarri C. Sotelo-Montes A.W. Brodie E. Cromwell K. Schreckenberg A.J. Simons (1997) Farmers’ use and management of tree germplasm: case studies from the Peruvian Amazon Basin A.J. Simons R. Kindt F. Place (Eds) Policy Aspects of Tree Germplasm Demand and Supply. Proceedings of an international workshop held in Nairobi, Kenya ICRAF Nairobi, Kenya 57–63
J.C. Weber C. Sotelo-Montes H. Vidaurre I.K. Dawson A.J. Simons (2001) ArticleTitleParticipatory domestication of agroforestry trees: an example from the Peruvian Amazon Dev. Practice 11 425–433 Occurrence Handle10.1080/09614520120066710
J.G.K. Williams A.R. Kuberlik K.J. Livak J.A. Rafalski S.V. Tingey (1990) ArticleTitleDNA polymorphisms amplified by arbitrary primers are useful as genetic markers Nucleic Acids Res. 18 6531–6535 Occurrence Handle1979162 Occurrence Handle1:CAS:528:DyaK3MXjslWmsA%3D%3D
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Lengkeek, A.G., Mwangi, A.M., Agufa, C.A.C. et al. Comparing genetic diversity in agroforestry systems with natural forest: a case study of the important timber tree Vitex fischeri in central Kenya. Agroforest Syst 67, 293–300 (2006). https://doi.org/10.1007/s10457-005-5830-6
- Genetic erosion
- Tree domestication
- Vitex keniensis