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Vessel tech: a high-accuracy pipeline for comprehensive mouse retinal vasculature characterization

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Abstract

Mouse retinal vasculature is a well-recognized and commonly used animal model for angiogenesis and microvascular remodeling. Morphological features of retinal vasculature reflect the vessel’s biological functions, and are critical in understanding the physiological and pathological process of vascular development and disease. Here we developed a comprehensive software, Vessel Tech, using retinal vasculature images of postnatal mice. This pipeline can automatically process retinal vascular images, reconstruct vessel network with high accuracy and assess global and local vascular characteristics based on the recent machine-learning techniques. The development of Vessel Tech provides a powerful tool for vascular biologists.

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Acknowledgements

We acknowledge Jay Hwang for editing the manuscript.

Funding

This work was supported by Jinjiang Pang’s Grants from the National Institutes of Health (R01 HL122777-05, R01 HL122777-06A1) and American Heart Association Innovative Project Award (19IPLOI34760446).

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Author notes

  1. Xuelin Wang and Guofu Zhu contributed equally to this paper

Authors and Affiliations

  1. Department of Biostatistics and Computational Biology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA

    Xuelin Wang & Zhengwu Zhang

  2. Department of Cardiology, Pan-Vascular Research Institute, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China

    Guofu Zhu

  3. Aab Cardiovascular Research Institute, Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA

    Shumin Wang, Jordan Rhen & Jinjiang Pang

Authors
  1. Xuelin Wang
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  2. Guofu Zhu
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  3. Shumin Wang
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  4. Jordan Rhen
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  5. Jinjiang Pang
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  6. Zhengwu Zhang
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Corresponding authors

Correspondence to Jinjiang Pang or Zhengwu Zhang.

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Wang, X., Zhu, G., Wang, S. et al. Vessel tech: a high-accuracy pipeline for comprehensive mouse retinal vasculature characterization. Angiogenesis 24, 7–11 (2021). https://doi.org/10.1007/s10456-020-09752-8

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