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RNA m6A methylation promotes the formation of vasculogenic mimicry in hepatocellular carcinoma via Hippo pathway

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A Correction to this article was published on 30 September 2022

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Abstract

Vasculogenic mimicry (VM) formed by aggressive tumor cells to mimic vasculogenic networks plays an important role in the tumor malignancy of HCC. However, the pathogenesis underlying VM is complex and has not been fully defined. m6A is a common mRNA modification and has many biological effects. However, the relationship between m6A and VM remains unclear. In this research, we found that m6A methyltransferase METTL3 in HCC tissues was positively correlated with VM. The m6A level of mRNA significantly increased in 3D cultured cells treated with VEGFa and was related to VM formation. Transcriptome sequencing analysis of 3D cultured cells with knockdown Mettl3 showed that the Hippo pathway was involved in m6A-mediated VM formation. Further mechanism research indicated that the m6A modification of YAP1 mRNA affected the translation of YAP1 mRNA. In conclusion, m6A methylation plays a key role in VM formation in HCC. METTL3 and YAP1 could be potential therapeutic targets via impairing VM formation in anti-metastatic strategies.

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Abbreviations

HCC:

Hepatocellular carcinoma

VM:

Vasculogenic mimicry

m6A:

N6-methyladenosine

VEGFa:

Vascular endothelial growth factor a

YAP1:

Yes associated protein 1

DEN:

Diethylnitrosamine

METTL3:

Methyltransferase-like 3

ALKBH5:

AlkB homolog 5

PAS:

Periodic acid–Schiff

IHC:

Immunohistochemistry

CDH5:

Cadherin 5

MMP2:

Matrix metallopeptidase 2

MMP9:

Matrix metallopeptidase 9

FN1:

Fibronectin 1

MeRIP:

Methylated RNA immunoprecipitation

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Acknowledgements

We are grateful to Prof. Chuan He, Prof. Jianhua Yang, and Prof. Jianjun Chen for providing us with the omics data of MeRIP. This study was supported by National Science and Technology Major Project (Grant No. 2018ZX09736005), the National Natural Science Foundation of China (Grant Nos. 81872374, 81972629, 81972746, 81703581), the Tianjin Science and Technology Project (Grant No. 19JCJQJC63200), and the Taishan Scholars Program of Shandong Province (Grant No. tsqn201909193)

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  1. Kailiang Qiao and Yantao Liu contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, 38 Tongyan Road, Haihe Education Park, Tianjin, China

    Kailiang Qiao, Yantao Liu, Zheng Xu, Haohao Zhang, Heng Zhang, Chao Zhang, Zhi Chang, Xinyan Lu, Zhongwei Li, Ce Luo, Cheng Yang & Tao Sun

  2. Tianjin Key Laboratory of Molecular Drug Research, Tianjin International Joint Academy of Biomedicine, Tianjin, China

    Kailiang Qiao, Yantao Liu, Zheng Xu, Haohao Zhang, Heng Zhang, Zhi Chang, Cheng Yang & Tao Sun

  3. Department of Pathology, Affiliated Hospital of Jining Medical University, Jining Medical University, No.89, Guhuai Road, Rencheng District, Jining, Shandong, China

    Yanrong Liu

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Contributions

KQ: acquisition, analysis and interpretation of data, drafting the manuscript. YL, HZ: acquisition of data, statistical analysis; ZX, ZC: acquisition of data; HZ, CZ: technical support; XL: acquisition of data; ZL: acquisition of data; CL: acquisition of data; YL: clinicopathological analysis, CY: study concept and design; TS: study concept and design, study supervision, analysis of data, drafting the manuscript.

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Correspondence to Tao Sun.

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HCC tissue microarrays from 75 and 96 cases were purchased from US Biomax for IHC. Each single tissue spot on every array slide was individually examined by certified pathologists according to the WHO published standards for diagnosis, classification, and pathological grading.

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Animal experiments were conducted in accordance with the National Institutes of Health Animal Use Guidelines. All experimental protocols were approved by the Institutional Animal Care and Use Committee at Tianjin International Joint Academy of Biomedicine.

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Qiao, K., Liu, Y., Xu, Z. et al. RNA m6A methylation promotes the formation of vasculogenic mimicry in hepatocellular carcinoma via Hippo pathway. Angiogenesis 24, 83–96 (2021). https://doi.org/10.1007/s10456-020-09744-8

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