Abstract
MicroRNAs (miRNAs) are single-stranded small non-coding RNAs, generally 18–25 nucleotides in length, that act as repressors of gene expression. miRNAs are encoded by independent genes or processed from a variety of different RNA species. So far, there is no evidence showing that the ribosomal DNA-hosted microRNA is implicated in vertebrate development. Currently, we found a highly expressed small RNA hosted in ribosomal DNA was predicted as a novel miRNA, named miR-ntu1, in zebrafish endothelial cells by deep sequencing analysis. The miRNA was validated by custom-designed Taqman PCR, Northern Blot, and in silico analysis. Furthermore, we demonstrated that miR-ntu1 played a crucial role in zebrafish angiogenesis via modulation of Notch signaling. Our findings provide a notable case that a miRNA hosted in ribosomal DNA is involved in vertebrate development.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China 81570447, 81870359; Natural Science Foundation from Jiangsu Province 17KJA180008, SWYY-048, and BK20180048.
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Shi, Y., Duan, X., Xu, G. et al. A ribosomal DNA-hosted microRNA regulates zebrafish embryonic angiogenesis. Angiogenesis 22, 211–221 (2019). https://doi.org/10.1007/s10456-019-09663-3
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DOI: https://doi.org/10.1007/s10456-019-09663-3