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The chicken chorioallantoic membrane model in biology, medicine and bioengineering

Angiogenesis volume 17, pages 779–804 (2014)Cite this article

Abstract

The chicken chorioallantoic membrane (CAM) is a simple, highly vascularized extraembryonic membrane, which performs multiple functions during embryonic development, including but not restricted to gas exchange. Over the last two decades, interest in the CAM as a robust experimental platform to study blood vessels has been shared by specialists working in bioengineering, development, morphology, biochemistry, transplant biology, cancer research and drug development. The tissue composition and accessibility of the CAM for experimental manipulation, makes it an attractive preclinical in vivo model for drug screening and/or for studies of vascular growth. In this article we provide a detailed review of the use of the CAM to study vascular biology and response of blood vessels to a variety of agonists. We also present distinct cultivation protocols discussing their advantages and limitations and provide a summarized update on the use of the CAM in vascular imaging, drug delivery, pharmacokinetics and toxicology.

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Abbreviations

AA:

Anti-angiogenic

AC:

Anti-cancer

ALA:

Aminolevulinic acid

AM:

Anti-microbial

AMD:

Age-related macular degeneration

BrdU:

Bromodeoxyuridine

CAM:

Chicken chorioallantoic membrane

CNV:

Subfoveal choroidal neovascularization

DDS:

Drug delivery systems

DPPC:

Dipalmitoylphosphatidylcholine

ECs:

Endothelial cells

ECM:

Extracellular matrix

GF:

Growth factor

HGF:

Hepatocyte growth factor

LC:

Langerhans cells

LF:

Lipid factor

MR:

Microbeam radiation

PCV:

Polypoidal choroidal vasculopathy

PDD:

Photodynamic diagnosis

PEG:

Polyethylene glycol

PLGA:

Poly(lactide-co-glycolide)

PpIX:

Protoporphyrin IX

Prox1:

Prospero homeobox protein 1

PS:

Photosensitizer

SMA:

Smooth muscle actin

VDA:

Vascular disrupting agents

VM:

Vasculogenic mimicry

YSM:

Yolk sac membrane

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Acknowledgments

The authors acknowledge Dr. Valentin Djonov for delivery of the unpublished scanning electron microscopy image (Fig. 10c) and Dr. Steven Menzler for the images shown in Fig. 10a, d.

Conflict of interest

The authors declare that they have no conflict of interest.

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Affiliations

  1. Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology (EPFL), 1015, Lausanne, Switzerland

    Patrycja Nowak-Sliwinska

  2. Department of Chemical and Biomolecular Engineering, UCLA, Los Angeles, CA, USA

    Tatiana Segura

  3. Molecular Biology Institute, UCLA, 615 Charles E. Young Drive South, Los Angeles, CA, 90095, USA

    M. Luisa Iruela-Arispe

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  1. Patrycja Nowak-Sliwinska
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  2. Tatiana Segura
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  3. M. Luisa Iruela-Arispe
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Correspondence to Patrycja Nowak-Sliwinska or M. Luisa Iruela-Arispe.

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Nowak-Sliwinska, P., Segura, T. & Iruela-Arispe, M.L. The chicken chorioallantoic membrane model in biology, medicine and bioengineering. Angiogenesis 17, 779–804 (2014). https://doi.org/10.1007/s10456-014-9440-7

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  • DOI: https://doi.org/10.1007/s10456-014-9440-7

Keywords

  • Angiogenesis
  • Anti-vascular therapies
  • Chicken chorioallantoic membrane
  • Bioengineering
  • Lymphangiogenesis
  • Organ transplantation
  • Physical forces
  • Tumor vasculature