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Duffy antigen/receptor for chemokines (DARC) attenuates angiogenesis by causing senescence in endothelial cells

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Abstract

Duffy antigen/receptor for chemokines (DARC), expressed on erythrocytes and post-capillary venular endothelial cells, selectively binds both CXC and CC chemokines. DARC binds ELR + angiogenic chemokines such as IL-8 (CXCL8). We show that the DARC on endothelial cells plays a direct role in regulating angiogenesis. MatrigelTM in vivo plug assay showed that there was more capillary formation in DARC knockout mice compared to wild type mice indicating that DARC attenuated angiogenic activity. In vitro angiogenic assay on MatrigelTM coated plates using DARC expressing stable human cerebro-microvascular endothelial cells (HCEC) showed that, although capillary formation in transfected cells started early within 4–8 h; capillary formation was attenuated within 12–24 h. Contrarily, mock transfected cells continued to show vascular capillary formation during that time without demonstrating any attenuation. Preincubation of DARC-expressing HCEC with monoclonal antibody (mAb-Fy6) against the N-terminal chemokine-binding domain of DARC increased capillary formation in vitro. Moreover, addition of excess IL-8 during incubation had the similar effect. DARC-expressing transfected endothelial cells underwent senescence in conditioned medium, whereas DARC non-expressing cells remained healthy. Interestingly, after several days in the conditioned medium, DARC expressing senescent cells started to initiate capillary formation; whereas capillary formed with DARC non-expressing cells remained the same. Our data evidently demonstrated that DARC on endothelial cells attenuated the angiogenic activity by causing senescence.

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Abbreviations

IL-8:

Interleukin 8

MCP-1:

Monocyte chemoattractant protein 1

RANTES:

Regulated on activation, normal T expressed and secreted

MIP:

Macrophage inflammatory protein

MGSA:

Melanocyte growth stimulatory activity

PF4:

Platelet factor 4

GRO:

Growth related oncogene

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Acknowledgment

We acknowledge the generous gift of the HCEC brain microvascular cell line by Drs. Muruganandam and Stanimirovic of the Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario. This study was supported by an intramural funding from New York Blood Center, NY.

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  1. Lili Xu

    Present address: Department of Medicine–Rheumatology, Physicians & Surgeons, Rheu-Box 89, Mail Code: 8, New York, NY, 11032, USA

Authors and Affiliations

  1. Stem Cell/Cellular Therapy, New York Blood Center, 310 East 67th Street, New York, NY, 10021, USA

    Lili Xu, Annette Ashkenazi & Asok Chaudhuri

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  1. Lili Xu
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Correspondence to Asok Chaudhuri.

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Xu, L., Ashkenazi, A. & Chaudhuri, A. Duffy antigen/receptor for chemokines (DARC) attenuates angiogenesis by causing senescence in endothelial cells. Angiogenesis 10, 307–318 (2007). https://doi.org/10.1007/s10456-007-9084-y

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  • DOI: https://doi.org/10.1007/s10456-007-9084-y

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