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A comparative study on the allergenicity, structure, and ultrastructure of two Acacia pollen grains in guinea pigs

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Abstract

Acacia saligna and Acacia victoria are native to Australia brought to and grown in Southern Iran. They have since had a widespread distribution and become native in Iran. As people of the region are exposed to the pollen grains from these plants during a 5 months period, this study aimed at investigating the allergenicity of their mature pollen grains. In addition, the structural and ultrastructural as well as the total protein content of the mature pollen grains were analyzed. Pollen grains of A. saligna and A. victoria were collected from the suburbs of Ahvaz, Iran. Pollen extracts (15 % concentration) were prepared in PBS (pH 7.2), and 4- to 6-week-old male guinea pigs (Hartly) were used for allergenicity tests. Skin tests showed a significant increase in flare diameter. Clinical tests also showed a significant change in the levels of eosinophils, neutrophils, and IgE. Comparative analysis of the allergenicity of A. saligna and A. victoria pollen grains revealed a higher IgE level in the latter. Comparative analysis of pollen characteristics was performed using both light and electron microscopy for sporoderm structure and ultrastructure, respectively. Twelve percent PAGE analysis of the total protein content of mature pollen grains showed a greater number of bands in A. victoria, while four bands were common in both species. In conclusion, this study demonstrates that both A. saligna and A. victoria pollen grains are allergenic, with a greater allergenicity of A. victoria pollen grains. In addition, the structural and ultrastructural as well as the total protein content of the mature pollen grains are revealed, and a potential protein allergen is proposed.

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References

  • Abbas, A. K., Lichtman, A. H., & Pillai, S. (2007). Properties and overview of immune responses. In: Cellular and molecular immunology (6th ed., p. 5). Philadelphia: Saunders Elsevier.

  • Aberg, N. (1989). Asthma and allergic rhinitis in Swedish conscripts. Clinical and Experimental Allergy, 19, 59–63.

    Article  CAS  Google Scholar 

  • Bener, A., Safa, W., Abdulhalik, S., & Lestringant, G. G. (2002). An analysis of skin prick test reactions in asthmatics in a hot climate and desert environment. Allergy Immunology (Paris), 34(8), 281–286.

    CAS  Google Scholar 

  • Björkstén, B., Clayton, T., Ellwood, P., Stewart, A., Strachan, D., & ISAAC Phase III Study Group. (2008). Worldwide time trends for symptoms of rhinitis and conjunctivitis: Phase III of the International Study of Asthma and Allergies in Childhood. Pediatric Allergy and Immunology, 19(2), 110–124.

    Article  Google Scholar 

  • Bradford, M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.

    Article  CAS  Google Scholar 

  • Caccavari, M. A. (2002). Pollen morphology and structure of Tropical and Subtropical American genera of the Piptadenia-group (Leguminosae: Mimosoideae). Grana, 41, 130–141.

    Article  Google Scholar 

  • Clot, B. (2003). Trends in airborne Pollen: An overview of 21 years of data in Neuchâtel (Switzerland). Aerobiologia, 19(3–4), 227–234.

    Article  Google Scholar 

  • D’Amato, G., Liccardi, G., D’Amato, M., & Holgate, S. (2005). Environmental risk factors and allergic bronchial asthma. Clinical and Experimental Allergy, 35, 113–1124.

    Article  Google Scholar 

  • De Zotti, R., & Gubian, F. (1996). Asthma and rhinitis in wooding workers. Allergy and Asthma Proceedings, 17(4), 199–203.

    Article  Google Scholar 

  • Erdthman, G. (1960). The acetolysis method. A revised description. Svensk Botanisk Tidskrift, 54, 556–564.

    Google Scholar 

  • Fang, R., Xie, S., & Wei, F. (2001). Pollen survey and clinical research in Yunnan, China. Aerobiologia, 17(2), 165–169.

    Article  CAS  Google Scholar 

  • Fereidouni, M., Hossini, R. F., Azada, F. J., Assarezadegana, M. A., & Varasteh, A. (2009). Skin prick test reactivity to common aeroallergens among allergic rhinitis patients in Iran. Allergologia et Immunopathologia, 37(2), 73–79.

    Article  Google Scholar 

  • Heslop-Harrison, J. (1966). Cytoplasmic continuities during spore formation in flowering plants. Endeavour, 25, 65–72.

    Article  Google Scholar 

  • Howlett, B. J., Hill, D. J., & Knox, R. B. (1982). Cross-reactivity between Acacia (wattle) and rye grass pollen allergens. Detection of allergens in Acacia (wattle) pollen. Clinical Allergy, 12(3), 259–268.

    Article  CAS  Google Scholar 

  • Irian, S., Majd, A., Hoseinizadeh, A., & Jonubi, P. (2012). A study on the allergenicity and ontogeny of Acacia farnesiana pollen grains in guinea pigs. Aerobiologia, doi:10.1007/s10453-012-9258-y.

    Google Scholar 

  • Knox, B., & Suphioglu, C. (1996). Enviromental and molecular biology of pollen allergens. Trend in Plant Science, 5, 156–164.

    Article  Google Scholar 

  • Kwaasi, A. A., Harfi, H. A., Parhar, R. S., Saleh, S., Collison, K. S., Panzani, R. C., et al. (2002). Cross-reactivities between date palm (Phoenix dactylifera L.) polypeptides and foods implicated in the oral allergy syndrome. Allergy, 57(6), 508–518.

    Article  CAS  Google Scholar 

  • Kwaasi, A. A., Parhar, R. S., Al-Mohanna, F. A., Harfi, H. A., Collison, K. S., & Al-Sedairy, S. T. (1998). Aeroallergens and viable microbes in sandstorm dust. Potential triggers of allergic and nonallergic respiratory ailments. Allergy, 53(3), 255–265.

    Article  CAS  Google Scholar 

  • Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685.

    Article  CAS  Google Scholar 

  • Majd, A., Chehregani, A., Moin, M., Gholami, M., Kohno, S., Nabe, T., et al. (2004). The effects of air pollution on structures, proteins and allergenicity of pollen grains. Aerobiologia, 20, 111–118.

    Article  Google Scholar 

  • Milton, S. J., & Hall, A. V. (1981). Reproductive biology of Australian acacias in the south-western Cape Province, South Africa. Transactions of the Royal Society of South Africa, 44, 465–487.

    Article  Google Scholar 

  • Mondal, A. K., Parui, S., Biswas, S. R., & Mandal, S. (1997). Identification of the allergenic proteins of Ipomoea fistulosa pollen: Partial characterized and sensitivity test. Grana, 36, 301–305.

    Article  Google Scholar 

  • Onishi, N., Kawamoto, S., Ueda, K., Yamanaka, Y., Katayama, A., Suzuki, H., et al. (2007). Dietary pulverized konjac glucomannan prevents the development of allergic rhinitis-like symptoms and IgE response in mice. Bioscience, Biotechnology, and Biochemistry, 71, 2551–2556.

    Article  CAS  Google Scholar 

  • Rothenberg, M. E. (2007). Eosinophils in the new millennium. The Journal of Allergy and Clinical Immunology, 119, 1321–1322.

    Article  Google Scholar 

  • Sheldon, J. M., Lovell, R. G., & Mathews, K. P. (1967). A manual of clinical allergy Pub W.B. Saunders Co. Phil, London. Cited by Shivpuri CR, Shivpuri DN: Re-evaluation of intradermal tests. Aspects of Allergy & Applied Immunology, 9, 205–214.

    Google Scholar 

  • Singh, M. B., Hough, T., Theerakulpisut, P., Avjioglu, A., Davies, S., Smith, P. M., et al. (1991). Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: Intracellular targeting to the amyloplast. Proceedings of the National Academy of Sciences of the United States of America, 88, 1384–1388.

    Article  CAS  Google Scholar 

  • Singh, M. B., Smith, P. M., & Knox, R. B. (1990). Molecular biology of rye-grass pollen allergens. Monographs in Allergy, 28, 101–120.

    CAS  Google Scholar 

  • Suliaman, F. A., Holmes, W. F., Kwick, S., Khouri, F., & Ratard, R. (1997). Pattern of immediate type hypersensitivity reactions in the Eastern Province, Saudi Arabia. Annals of Allergy, Asthma & Immunology, 78(4), 415–418.

    Article  CAS  Google Scholar 

  • Tantawy, M. E., Khalifa, S. F., Hamed, K. A., & Elazab, H. M. (2005). Palynological Study on Some Taxa of Mimosoideae (Leguminosae). International Journal of Agriculture & biology, 6, 857–868.

    Google Scholar 

  • Webb, D. B., Wood, P. J., & Smith, J. (1980). A guide to species selection for tropical and sub-tropical plantations. Tropical forestry papers no. 15. Oxford, UK: Commonwealth Forestry Institute (E, S available; F under preparation).

  • Wood-Baker, R., & Markos, J. (1997). Occupational asthma due to Blackwood (Acacia Melanoxylon). Australian and New Zealand Journal of Medicine, 27(4), 452–453.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

    Saeed Irian, Ahmad Majd, Aboozar Hoseinizadeh & Parisa Jonubi

Authors
  1. Saeed Irian
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  2. Ahmad Majd
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  3. Aboozar Hoseinizadeh
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  4. Parisa Jonubi
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Correspondence to Saeed Irian.

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Irian, S., Majd, A., Hoseinizadeh, A. et al. A comparative study on the allergenicity, structure, and ultrastructure of two Acacia pollen grains in guinea pigs. Aerobiologia 29, 333–339 (2013). https://doi.org/10.1007/s10453-012-9283-x

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  • DOI: https://doi.org/10.1007/s10453-012-9283-x

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