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Birch pollen allergen exposure: profiles, sources and characteristics. Settled dust and aeroallergens in indoor and outdoor environments of Finnish homes

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Abstract

The main source for indoor birch pollen (BP) allergens is outdoor particles, which are carried indoors mainly by people or pets and less likely via open windows and doors. So far, BP allergens have been shown by ELISA in the indoor air or in the settled dust, but these techniques have not enabled a reliable analysis of the respirable-sized fraction of air particles allergic subjects are exposed to. The aims of this study were to measure the airborne personal BP allergen exposure indoors and outdoors, to study the particle size of inhaled BP allergens and to analyse the allergen concentration of settled dust in relation to personal airborne allergen load. The air samples were collected before, during and after the BP season using a nasal air sampler, and the samples of settled dust were collected by a vacuum cleaner with a special collection device. BP allergens collected by nasal samplers were detected by the HALOgen immunoassay using birch pollen specific human IgE and rabbit IgG antibodies to BP, and the results were compared to IgG-ELISA used for detecting BP allergens in indoor settled dust and outdoor air. The highest concentrations of BP antigenic activity in settled dust were in the entrance corridor (next to the main front door) decreasing substantially from outdoors to indoors. Significant personal exposure to the airborne particles containing BP allergens outdoors was shown by the HALOgen immunostaining, before, during and after the BP season. During the BP season, the large, outdoor airborne particles carrying BP allergens were composed mainly of the pollen grains (75%). However, outside the pollen season, only a few large particles with stained (allergen) halos were found, and only 50% of those were pollen grains. In the indoor air, stained large and small particles appeared mainly during the pollen season and remained detectable until the end of the 6-week follow-up period. The results of the indoor settled dust BP antigenic activity detected by IgG-ELISA showed a good correlation with those of the HALOgen immunostaining. Human IgE detected BP grains with halos containing BP allergens from indoor air during the pollen season but none after the season. Higher sensitivity of the HALOgen™ assay was obtained using rabbit IgG antiserum, which revealed both small (5–20 μm) and large (>20 μm) particles with halos both before, during, and outside the peak BP season. Therefore, it is unlikely that the birch allergy symptoms indoors, after the pollen season, would be due to intact airborne pollen grains, but rather are due to other small particles carrying airborne BP allergens. A probable source of these particles is settled dust, which has been carried indoors by people or pets.

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References

  • Buters, J. T. M., Weichenmeier, S., Ochs, S., Pusch, G., Kreyling, W., Boere, A. J. F., et al. (2010). The allergen Bet v 1 in fractions of ambient air deviates from birch pollen counts. Allergy, 65, 850–858.

    Article  CAS  Google Scholar 

  • El-Ghazaly, G., & Gafström, E. (1995). Morphological and histochemical differentiation of the pollen wall of Betula pendula Roth. during dormancy up to anthesis. Protoplasma, 187, 88–102.

    Article  Google Scholar 

  • El-Ghazaly, G., Nakamura, S., Takahashi, Y., Cresti, M., Walles, B., & Milanesi, C. (1996). Localization of the major allergen Bet v 1 in Betula pollen using monoclonal labelling. Grana, 35, 369–374.

    Article  Google Scholar 

  • Graham, J. A. H., Pavlicek, P. K., Sercombe, J. K., Xavier, M. L., & Tovey, E. R. (2000). The nasal air sampler: A device for sampling inhaled aeroallergens. Annals of Allergy, Asthma & Immunology, 84, 599–604.

    Article  CAS  Google Scholar 

  • Green, B. J., Yli-Panula, E., & Tovey, E. R. (2006). Halogen immunoassay, a new method for the detection of sensitization to fungal allergens; comparisons with conventional techniques. Allergology International, 55(2), 131–139.

    Article  CAS  Google Scholar 

  • Haahtela, T. M. K. (1979). The prevalence of allergic conditions and immediate skin test reactions among Finnish adolescents. Clinical Allergy, 9, 53–60.

    Article  CAS  Google Scholar 

  • Hirst, J. M. (1952). An automatic volumetric spore tarp. Annals of Applied Biology, 36, 257–265.

    Article  Google Scholar 

  • Holmquist, L., & Vesterberg, O. (1999). Quantification of birch and grass pollen allergens in indoor air. Indoor air, 9, 85–91.

    Article  CAS  Google Scholar 

  • Holmquist, L., Weiner, J., & Vesterberg, O. (2001). Airborne birch and grass pollen allergens in street-level shops. Indoor Air, 11, 241–245.

    Article  CAS  Google Scholar 

  • Hugg, T., & Rantio-Lehtimäki, A. (2007). Indoor and outdoor pollen concentrations in private and public spaces during the Betula pollen season. Aerobiologia, 23, 119–129.

    Article  Google Scholar 

  • Hugg, T., Yli-Panula, E., & Rantio-Lehtimäki, A. (2011). Pollen and pollen allergens in indoor environments. In B. Flannigan, R. Samson, & J. A. Miller (Eds.), In microorganisms in home and indoor work environments: Diversity, health impacts, investigation and control (2nd ed., pp. 45–53). Boca Raton: CRC press.

    Google Scholar 

  • Jarolim, E., Rumpold, H., Endler, A. T., Ebner, H., Breitenbach, M., Scheiner, O., et al. (1989). IgE and IgG antibodies of patients with allergy to birch pollen as tools to define the allergen profile of Betula verrucosa. Allergy, 44, 385–395.

    Article  CAS  Google Scholar 

  • Kauppinen, E. I., Jäppinen, A. V. K., Hillamo, R. E., Rantio-Lehtimäki, A. H., & Koivikko, A. S. (1989). A static particle size selective bioaerosol sampler for the ambient atmosphere. Journal of Aerosol Science, 20, 829–833.

    Article  CAS  Google Scholar 

  • Laatikainen, T., von Hertzen, L., Koskinen, J. P., Mäkelä, M. J., Jousilahti, P., Kosunen, T. U., et al. (2011). Allergy gap between Finnish and Russian Karelia on increase. Allergy, 66, 886–892.

    Article  Google Scholar 

  • Matikainen, E., & Rantio-Lehtimäki, A. (1998). Semiquantitative and qualitative analysis of pre-seasonal airborne BP allergens in different particle sizes—Background information for allergen reports. Grana, 37, 293–297.

    Article  Google Scholar 

  • O’Meara, T., De Lucca, S., Sporik, R., Graham, A., & Tovey, E. (1998). Detection of inhaled cat allergen. Lancet, 351, 1488–1489.

    Article  Google Scholar 

  • O’Meara, T., & Tovey, E. (1999). Monitoring personal allergen exposure. Clinical Reviews in Allergy and Immunology, 18, 341–395.

    Article  Google Scholar 

  • Pehkonen, E., & Rantio-Lehtimäki, A. (1994). Variations in airborne pollen antigenic particles caused by meteorologic factors. Allergy, 49, 472–477.

    Article  CAS  Google Scholar 

  • Pehkonen, E., Rantio-Lehtimäki, A. & Yli-Panula, E. (1993). Submicroscopic antigenic particles in outdoor air: A contamination source if indoor air. In P. Kalliokoski, M. Jantunen & O. Seppänen (Eds.), Proceedings 6th Int. Conf. Indoor Air Quality Climate 4 (pp. 231–236). Gummerus, Jyväskylä.

  • Poulos, L., O′Meara, T., Sporik, R., & Tovey, E. (1999). Detection of inhaled Der p 1. Clinical & Experimental Allergy, 29, 1232–1238.

    Article  CAS  Google Scholar 

  • Rantio-Lehtimäki, A., Viander, M., & Koivikko, A. (1994). Airborne birch pollen antigens in different particle sizes. Clinical & Experimental Allergy, 24, 23–28.

    Article  Google Scholar 

  • Razmovski, V., O`Meara, T., Taylor, D. J. M., & Tovey, E. (2000). A new method for simultaneous immunodetection and morphologic identification of individual sources of pollen allergens. Journal Allergy Clinical Immunology, 105, 725–731.

    Article  CAS  Google Scholar 

  • Rehnström, A., Karlsson, A. S., & Tovey, E. (2002). Nasal air sampling used for the assessment of occupational allergen exposure and efficacy of respiratory protection. Clinical & Experimental Allergy, 32, 1769–1775.

    Google Scholar 

  • Spieksma, F. T. M., & Nikkels, A. H. (1999). Similarity in seasonal appearance between atmospheric birch-pollen grains and allergen in paucimicronic, size-frationated ambient aerosol. Allergy, 54, 235–241.

    Article  CAS  Google Scholar 

  • Swoboda, I., Dang, T. C. H., Heberle-Bors, E., & Vicente, O. (1995). Expression of Bet v I, the major birch pollen allergen, during anther development. An in situ hybridization study. Protoplasma, 187, 103–110.

    Article  CAS  Google Scholar 

  • Tovey, E. R., Taylor, D. J. M., Graham, A. H., O’Meara, T., Lovborg, U., Jones, A., et al. (2000). New immunodiagnostic system. Aerobiologia, 16, 113–118.

    Article  Google Scholar 

  • Yli-Panula, E., & Ahlholm, J. (1998). Prolonged antigenic activity of birch and grass pollen in experimental conditions. Grana, 37, 180–184.

    Article  Google Scholar 

  • Yli-Panula, E., & Rantio-Lehtimäki, A. (1994). Antigenic activity in settled dust outdoors; a study on birch pollen antigens. Grana, 33, 177–180.

    Article  Google Scholar 

  • Yli-Panula, E., & Rantio-Lehtimäki, A. (1995). Birch pollen antigenic activity of settled dust in rural and urban homes. Allergy, 50, 303–307.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

First of all I am grateful to Docent Auli Rantio-Lehtimäki who made this study a reality by providing the facilities at the Aerobiology Unit. I also want to thank the Aerobiology Group for collecting the outdoor airborne SSBAS and Burkard samples for me. Warm thanks go to Katja Järvinen for collecting the indoor and outdoor settled dust samples in 1998. I also want thank the three allergic subjects who collected the nasal air samples. Finally, many thanks go to Drs. Tim O′Meara, Euan Tovey, Timo Vanto and Markku Viander for the fruitful discussions about the analysing methods.

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Authors and Affiliations

  1. Department of Teacher Education, University of Turku, Assistentinkatu 5, 20014, Turku, Finland

    E. Yli-Panula

  2. Aerobiology Unit, University of Turku, Turku, Finland

    E. Yli-Panula

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  1. E. Yli-Panula
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Correspondence to E. Yli-Panula.

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Yli-Panula, E. Birch pollen allergen exposure: profiles, sources and characteristics. Settled dust and aeroallergens in indoor and outdoor environments of Finnish homes. Aerobiologia 28, 453–465 (2012). https://doi.org/10.1007/s10453-012-9248-0

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