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Aerobiologia

, Volume 26, Issue 3, pp 171–184 | Cite as

Assessment of bioaerosols at a concentrated dairy operation

  • Robert S. DunganEmail author
  • April B. Leytem
  • Sheryl A. Verwey
  • David L. Bjorneberg
Original paper

Abstract

Increased bioaerosol loadings in downwind plumes from concentrated animal feeding operations (CAFOs) may increase the risk for allergy and infection in humans. In this study, we monitored airborne concentrations of culturable bacteria and fungi at upwind (background) and downwind sites at a 10,000 milking cow dairy over the course of a year. The average bacterial concentrations at the upwind site were 8.4 × 103 colony forming units (CFU) m−3 and increased to 9.9 × 105 CFU m−3 at the downwind edge of the cattle lots, decreasing to 6.3 × 104 CFU m−3 200 m farther downwind. At the same sites, the average fungal concentrations were 515, 945, and 1,010 CFU m−3, respectively. Significant correlations between the ambient weather conditions and airborne fungal and bacterial concentrations were identified. Sequence analysis of PCR-amplified DNA from bacterial clones and fungal isolates revealed genus and species level differences between upwind and downwind sites. Although we could not cultivate gram-negative bacteria, bacterial clones at downwind sites identified as being gram-negative matched with the following genera: Acinetobacter, Bradyrhizobium, Escherichia, Idiomarina, Methylobacterium, Ralstonia, and Novosphingobium. Fungal isolates from downwind matched with the following genera: Acremonium, Alternaria, Ascomycte, Aspergillus, Basidiomycete, Cladosporium, Davidiella, Doratomyces, Emericella, Lewia, Onygenales, Penicillium, Rhizopus, and Ulocladium. None of the bacterial and fungal sequence matches were affiliated with genera and species known to be pathogenic to humans. Overall, the data suggest that exposure to bioaerosols in the downwind environment decreases with increasing distance from the open-lot dairy.

Keywords

Airborne bacteria and fungi Bioaerosols CAFOs Dairy Impaction Impingement Polymerase chain reaction 

Notes

Acknowledgments

We would like to thank numerous individuals at the dairy for accommodating us during this long study. We also thank the following individuals: Susie Hansen, Myles Miller, Patsy Heinemann, and Ann Pool.

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Copyright information

© US Government 2010

Authors and Affiliations

  • Robert S. Dungan
    • 1
    Email author
  • April B. Leytem
    • 1
  • Sheryl A. Verwey
    • 1
  • David L. Bjorneberg
    • 1
  1. 1.Northwest Irrigation and Soils Research Laboratory, Agricultural Research ServiceUS Department of AgricultureKimberlyUSA

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