Abstract
Nano mesoporous molecular sieve MCFs (mesocellular foams) was synthesized successfully through hydrothermal method. Then bovine hemoglobin was successfully immobilized into the mesoporous molecular sieve MCFs, the immobilized enzyme was 50.27 mg g−1. The desirable adsorbate/adsorbent, Hb/MCFs, (mass ratio, m/m) for 3/50 was achieved by adjusting adsorption conditions. The obtained materials were characterized using various techniques such as the powder XRD, Fourier transform infrared spectroscopy, UV–Vis solid diffuse reflectance spectroscopy (SDRS), photoluminescence spectrum and N2 adsorption desorption study at 77 K. The results showed that hemoglobin had been immobilized successfully inside the channels of mesoporous MCFs. Scanning electron microscopy was used to confirm the average particle diameters of MCFs-Hb and (CH3-MCFs)-Hb. And the results showed that the average particle diameters of MCFs-Hb and (CH3-MCFs)-Hb were 2212 ± 50 nm and 2219 ± 50 nm, respectively. The adsorption process of MCFs/(CH3-MCFs) adsorbing hemoglobin enzyme was in accordance with pseudo-second-order kinetic equation. The process of MCFs/(CH3-MCFs) adsorbing hemoglobin enzyme belonged to an exothermic reaction which was a spontaneous reaction. Freundlich model described the adsorption of hemoglobin by MCFs/CH3-MCFs well. The desorption process of MCFs-Hb/(CH3-MCFs)-Hb composite was studied in the sodium hydroxide solution (0.1 mol L−1), and equilibrium was reached at 5 h. The desorption rates were 72.58% and 69.67%, respectively. The luminescence spectra showed that the obtained composites had the light-emitting properties.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barett, E.P., Joyner, L.G., Halenda, P.P.: The determination of porevolume and area distributions in porous substances. I. Computation from nitrogen isotherms. J. Am. Chem. Soc. 73, 373–380 (1951)
Brumauuer, S., Emmett, P.H., Teller, E.: Adsorption of gases in multimolecular layers. J. Am. Chem. Soc. 60, 309–319 (1938)
Coma, A., Navarro, M.T., Pariente, J.P.: Synthesis of an ultralarge pore titanium silicate isomorphous to MCM-41 and its application as catalyst for selective oxidation of hydrocarbons. Chem. Soc. Chem. Common 142(1), 147–148 (1994)
Dai, Z., Liu, S., Ju, H., Chen, H.: Direct electron transfer and enzymatic activity of hemoglobin in a hexagonal mesoporous silica matrix. Biosens. Bioelecron. 19, 861–867 (2004)
Hartmann, M.: Ordered mesoporous materials for bioadsorption and biocatalysis. Chem. Mater. 17, 4577–4593 (2005)
Hernandez-Maldonado, J., Yang, R.T., Cannella, W.: Desulfurization of liquid fuels by adsorption via π-complexation with Cu(I)-Y zeolites. Ind. Eng. Chem. Res. 43, 6142–6149 (2004)
Ji, L., Katiyar, A., Pinto, N.G., Jaroniec, M., Smirniotis, P.G.: Al-MCM-41 sorbents for bovine serum albumin: relation between Al content and performance. Microporous Mesoporous Mater. 75, 221–229 (2004)
Laveille, P., Falcimaigne, A., Chamouleau, F., Renard, G., Drone, J., Fajual, F., Pulvin, S., Thomas, D., Bailly, C., Galarneau, A.: Hemoglobin immobilized on mesoporous silica as effective material for the removal of polycyclic aromatic hydrocarbons pollutants from water. New J. Chem. 34, 2153–2165 (2010)
Li, J., Zhou, L., Han, X., Hu, J., Liu, H., Xu, J.: Direct electrochemistry of hemoglobin immobilized on siliceous mesostructured cellular foam. Sens. Actuator B Chem. 138, 545–549 (2009)
Mody, H.N., Mody, K.H., Jasra, R.V., Shin, H.J., Ryong, R.: Catalytic activity of an immobilized a-amylase on mesoporous silicas. Indian J. Chem. Sect. 41, 1795–1803 (2002)
Nguyen, T.P.B., Lee, J.W., Shim, W.G., Moon, H.: Synthesis of functionalized SBA-15 with ordered large pore size and its adsorption properties of BSA. Microporous Mesoporous Mater. 110, 560–569 (2008)
Russo, P.A., Carrott, M.M.L.R., Mourao, P.A.M., Carrott, P.J.M.: Tailoring the surface chemistry of mesocellular foams for protein adsorption. Colloid Surf. A 386, 25–35 (2011)
Schmidt, W.P., Glinka, C.J., Stucky, G.D.: Microemulsion templates for mesoporous silica. Langmuir 16, 356–361 (2000)
Schmidt-Winkel, P., Lukens, W.W., Zhao, D.Y., Yang, P., Chmelka, B.F., Stucky, G.D.: Mesocellular siliceous foams with uniformly sized cells and windows. J. Am. Chem. Soc. 121, 254–255 (1999)
Song, S.W., Hidajat, K., Kawi, S.: Functionalized SBA-15 materials as carriers for controlled drug delivery: influence of surface properties on matrix–drug interactions. Langmuir 21, 9568–9575 (2005)
Sousa, A., Sousa, E.M.B.: Optimization of drying process of Zea mays malt to use as alternative source of amylolytics enzymes. Braz. Arch. Biol. Technol. 48, 243–246 (2005)
Urabe, Y., Shiomi, T.: Encapsulation of hemoglobin in mesoporous silica (FSM)-enhanced thermal stability and resistance to denaturants. Chem. Biol. Chem. 8, 668–674 (2007)
Wang, Q.S., Zhai, Q.Z., Luo, J.: Spectrophotometric determination of protein with DBC-chlorophosphonazo. Asian J. Chem. 25, 5470–5472 (2013)
Yu, J., Zhao, T., Zhao, F., Zeng, B.: Direct electron transfer of hemoglobin immobilized in a mesocellular siliceous foams supported room temperature ionic liquid matrix and the electrocatalytic reduction of H2O2. Electrochim. Acta 53, 5760–5765 (2008)
Zhai, Q.Z., Wang, Q., Zhang, X.X.: Preparation and characterization of methylated SBA-15. J. Chin. Ceram. Soc. 37, 531–535 (2009)
Zhang, L., Zhang, Q., Li, J.H.: Direct electrochemistry and electrocatalysis of hemoglobin immobilized in bimodal mesoporous silica and chitosan inorganic–organic hybrid film. Electrochem. Commun. 9, 1530–1535 (2007)
Acknowledgements
The research was financially supported by Young Teacher’s Foundation of Changchun University of Science and Technology, China (Project No: XQNJJ-2014-14) and the Natural Science Foundation of Jilin Province, P. R. China (Grant Number 201215146). The authors wish to acknowledge the financial help.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no competing interests.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Wang, QS., Song, CQ. & Zhai, QZ. Study on the immobilization of hemoglobin by nano mesoporous MCFs and its optical properties. Adsorption 26, 863–878 (2020). https://doi.org/10.1007/s10450-019-00139-w
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10450-019-00139-w