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Expanded bed adsorption of albumin and immunoglobulin G from human serum onto a cation exchanger mixed mode adsorbent

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Abstract

Adsorption of human serum albumin (HSA) and immunoglobulin G (IgG), two most relevant proteins in blood serum were measured separately on a new mixed-mode adsorbent (MabDirect MM), especially designed for expanded bed adsorption process. Expanded bed behavior characterization by residence time distribution experiments for different columns (Streamline 50 for HSA study, Omnifit 66/20 and XK 16/20 for IgG study) are presented together with breakthrough adsorption of HSA and IgG for different conditions and compared with simulation results by a mathematical model. Regarding HSA adsorption, all four experiments were conducted at the same buffer solution (pH 5.0 without salt), and it was obtained a dynamic binding capacity of 8.9, 9.7, 7.5 and 7.0 mg·gdry−1 (24.8, 27.0, 21.0 and 19.5 mg·cm−3) at 10% of breakthrough, corresponding to a 41, 39, 38 and 30% of the saturation capacity for runs 1–4, respectively. The results from the simulation of the mathematical model fitted well with the breakthrough experiments. Elution stage was optimized by lowering flow rate and changing the buffer solution pH and NaCl concentration. Concerning IgG adsorption, for IgG feed concentration of 0.53 g·dm−3 in 20 mM citrate buffer, pH 5.0 with 0.4 M NaCl, at 2.2 cm3·min−1, it was obtained a DBC of 3.3 mg·gdry−1 (9.1 mg·cm−3) at 10% of breakthrough, representing 22% of the saturation capacity. While for a feed concentration of 0.11 g·dm−3 of IgG in the same buffer solution, at 6 cm3·min−1, it was obtained a DBC of 2.7 mg·gdry−1 (7.4 mg·cm−3) at 10% of breakthrough, representing 34% of the saturation capacity.

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Acknowledgements

The authors would like to thank Upfront Chromatography A/S for kindly providing the adsorbent. This work is a result of project “AIProcMat@N2020—Advanced Industrial Processes and Materials for a Sustainable Northern Region of Portugal 2020”, with the reference NORTE-01-0145-FEDER-000006, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and of Project POCI-01-0145-FEDER-006984—Associate Laboratory LSRE-LCM funded by ERDF through COMPETE2020—Programa Operacional Competitividade e Internacionalização (POCI)—and by national funds through FCT—Fundação para a Ciência e a Tecnologia.

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  1. Laboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials (LSRE-LCM), Department of Chemical Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal

    Pedro Ferreira Gomes, José Miguel Loureiro & Alírio E. Rodrigues

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  1. Pedro Ferreira Gomes
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  2. José Miguel Loureiro
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Correspondence to Alírio E. Rodrigues.

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Gomes, P.F., Loureiro, J.M. & Rodrigues, A.E. Expanded bed adsorption of albumin and immunoglobulin G from human serum onto a cation exchanger mixed mode adsorbent. Adsorption 24, 293–307 (2018). https://doi.org/10.1007/s10450-018-9940-7

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