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Comparing multi-column chromatographic processes for purifying monosaccharides part I: A simplified approach


This work proposes a methodology for comparing different multi-column chromatographic processes (SMB, ISMB, SSMB) for purifying monosaccharides. The first step of the methodology consists in determining the flow rates associated with the different processes assuming that the columns are infinitely efficient. This allows deriving “idealized” operating conditions. In a second step, using the “idealized” conditions, the behavior of the real systems associated with columns of finite efficiencies are simulated with ChromWorks™ and compared. It is shown that the SMB performs better than single column elution chromatography, and that, for the application selected, ISMB and SSMB are very similar and perform better than SMB.

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\(\text{BV}\) :

Volume normalized by the column volume

\(\bar{K}\) :

Henry’s coefficient

\(m\) :

Ratio of fluid flow rate to solid flowrate (TMB)

N :

Number of plates

\({{P}_{Fru}}\) :

Fructose purification factor

\(Q\) :

Fluid flow rate

\(\bar{Q}\) :

Solid flowrate (TMB)

\({{u}_{e}}\) :

Interstitial fluid velocity

\({{V}_{col}}\) :

Column volume

\({{X}_{Fru}}\) :

Fructose purity

\(\Delta {{t}_{inj}}\) :

Injection time

\(\Delta T\) :

Shift period

\(\Delta V\) :

Volume injected

\({{\varepsilon }_{e}}\) :

Extragranular porosity

\({{\theta }_{Fru}}\) :

Fructose recovery

\({{\Phi }_{Fru}}\) :

Fructose productivity

\({{\Upsilon }_{Fru}}\) :

Specific eluent consumption


Equilibrium model

ec, tmb, smb, ismb, ssmb:

Refer to chromatographic processes


Zones in TMB or SMB

E, F, Ext, Raf:

Eluent, Feed, Extract, Raffinate


Bed volume


Intermittent SMB


Simulated moving bed


Sequential SMB


True moving Bed


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Correspondence to Roger-Marc Nicoud.

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David, L., Yun, J. & Nicoud, RM. Comparing multi-column chromatographic processes for purifying monosaccharides part I: A simplified approach. Adsorption 23, 577–591 (2017).

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  • Sugars
  • Chromatography
  • Simulation
  • Simulated Moving Bed
  • Ion exchange