Abstract
Influence of pH of the BSA solutions on velocity of the rising bubbles, stability of foams, and properties of single foam and wetting films was studied. It was found that the solution pH affected significantly the BSA surface activity and properties of the protein adsorption layer under dynamic and static conditions. At pH close to the isoelectric point (pHIEP=4.8) the BSA showed the highest surface activity. The equilibrium microscopic foam films of thicknesses of 64–80 nm, depending on the BSA concentration, were obtained at pH=5.8. Under dynamic conditions the bubble rising velocity was reduced in a highest degree and the foam formed were most stable at the solutions pH-5.8 and 4.8. Lowering the bubble velocity shows that the BSA adsorption layer was formed, which retarded fluidity of the bubble surface. When the solution pH was significantly lower (pH=3.9) or much higher (pH=10) than the pHIEP then the BSA practically had no influence on the bubble velocity and the foam stability was drastically reduced. Moreover, the pH variations affected also the time of the three-phase contact (TPC) formation on mica surface covered by the BSA adsorption layers. These pH dependent changes in the BSA surface activity indicate that the BSA linear conformers, existing at pH far away from the pHIEP, have much higher affinity to aqueous phase resulting from higher net electrical charge present over the extended BSA molecule conformers.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Berthold, A., Schuber, H., Brandes, N., Kroh, L., Miller, R.: Behaviour of BSA and of BSA-derivatives at the air/water interface. Colloids Surf. A 301, 16–22 (2007)
Cascao Pereira, L.G., Johansson, Ch., Radke, C.J., Blanch, H.W.: Surface forces and drainage kinetics of protein-stabilized aqueous films. Langmuir 19, 7503–7513 (2003b)
Cascao Pereira, L.G., Theodoly, O., Blanch, H.W., Radke, C.J.: Dilatational rheology of BSA conformers at the air/water interface. Langmuir 19, 2349–2356 (2003a)
Clark, D.C., Coke, M., Mackie, A.R., Pinder, A.C., Wilson, D.R.: Molecular diffusion and thickness measurements of protein-stabilized thin liquid films. J. Colloid Interface Sci. 138, 207–219 (1990)
Clift, R., Grace, J.R., Weber, N.E.: Bubbles, Drops and Particles. Academic Press, New York (1978), Chap. 9
Cohen, R., Exerowa, D., Kolarov, T., Yamanaka, T., Muller, V.: Thickness transitions in lysolecithin foam films. Colloids Surf. A 65, 201–209 (1992)
Curvale, R., Masuelli, M., Padilla, A.P.: Intrinsic viscosity of bovine serum albumin conformers. Int. J. Biol. Macromol. 42, 133–137 (2008)
Dukhin, S.S., Kretzschmar, G., Miller, R.: Dynamics of Adsorption at Liquid Interfaces. Theory, Experiments, Application. Elsevier, Amsterdam (1995)
Dukhin, S.S., Miller, R., Loglio, G.: Physico-chemical hydrodynamics of rising bubble. In: Möbius, D., Miller, R. (eds.) Drops and Bubbles in Interfacial Research, pp. 367–433. Elsevier, New York (1998)
Exerowa, D., Kruglyakov, P.: In: Mobius, D., Miller, R. (eds.) Foam, and Foam films Elsevier, Amsterdam (1998)
Graciaa, A., Morel, G., Saulner, P., Lachaise, J., Schechter, R.S.: The ζ-potential of gas bubbles. J. Colloid Interface Sci. 172, 131–136 (1995)
Graham, D.E., Philips, M.C.: Proteins at liquid interfaces: I. Kinetics of adsorption and surface denaturation. J. Colloid Interface Sci. 70(3), 403–414 (1979a)
Graham, D.E., Philips, M.C.: Proteins at liquid interfaces: II. Adsorption isotherms. J. Colloid Interface Sci. 70(3), 415–426 (1979b)
Jachimska, B., Wasilewska, M., Adamczyk, Z.: Characterization of globular protein solutions by dynamic light scattering, electrophoretic mobility and viscosity measurements. Langmuir 24, 6866–6872 (2008)
Khan, M.Y.: Direct evidence for the involvement of domain III in the N–F transition of bovine serum albumin. Biochem. J. 236, 307–310 (1986)
Krasowska, M., Kolasinska, M., Warszynski, P., Malysa, K.: Influence of polyelectrolyte layers deposited on mica surface on wetting film stability and bubble attachment. J. Phys. Chem. C 111, 5743–5749 (2007)
Krasowska, M., Malysa, K.: Kinetics of bubble collision and attachment to hydrophobic solids: I. Effect of surface roughness. Int. J. Miner. Process. 81, 205–216 (2007)
Krzan, M., Lunkenheimer, K., Malysa, K.: On the influence of the surfactant’s polar group on the local and terminal velocities of bubbles. Colloids Surf. A 50, 431–441 (2004)
Krzan, M., Malysa, K.: Profiles of local velocities of bubbles in n-butanol, n-hexanol and n-nonanol solutions. Colloids Surf. A 207, 279–291 (2002)
Krzan, M., Zawala, J., Malysa, K.: Development of steady state adsorption distribution over interface of a bubble rising in solutions of n-alkanols (C5, C8) and n-alkyltrimethylammonium bromides (C8, C12, C16). Colloids Surf. A 298, 42–51 (2007)
Lee, J.S.H., Li, D.: Electroosmotic flow at a liquid–air interface. Microfluid. Nanofluid. 2, 361–365 (2006)
Levich, V.G.: Physicochemical Hydrodymanics. Englewood Cliffs, Prentice-Hall (1962)
Liao, Y., McLaughlin, J.B.: Bubble motion in aqueous surfactant solutions. J. Colloid Interface Sci. 224, 297–310 (2000)
Lu, J.R., Su, T.J., Thomas, R.K.: Structural conformation of bovine serum albumin layers at the air–water interface studied by neutron reflection. J. Colloid Interface Sci. 213, 426–437 (1999)
Lunkenheimer, K., Malysa, K., Wienskol, G., Baranska, M.: European patent bulletin, 16.01.2008 (Art. 97(3) EPC), European Patent No. 1 416 261 (03 024 885.0)
Lunkenheimer, K., Malysa, K., Winsel, K., Geggel, K., Siegel, St.: Novel method and parameters for testing and characterization of foam stability. Langmuir 26(6), 3883–3888 (2010)
Magdassi, S., Kamyshny, A.: Introduction—surface activity and functional properties of proteins. In: Magdassi, S. (ed.) Surface Activity of Proteins—Chemical and Physicochemical Modifications, pp. 1–38. Marcel Dekker, New York (1996)
Malysa, K., Krakowska, M., Krzan, M.: Influence of surface active substances on bubble motion and collision with various interfaces. Adv. Colloid Interface Sci. 114–115, 205–225 (2005)
Malysa, K., Lunkenheimer, K.: Foams under dynamic conditions. Curr. Opin. Colloid Interface Sci. 13, 150–162 (2008)
Okubo, T., Kobayashi, K.: Surface tension of biological polyelectrolyte solutions. J. Colloid Interface Sci. 205, 433–442 (1998)
Patino, J.M.R., Nino, M.R.R., Sánchez, C.C.: Physico-chemical properties of surfactant and protein films. Curr. Opin. Colloid Interface Sci. 12, 187–195 (2007)
Platikanov, D., Exerowa, D.: Thin films. In: Lyklema, H. (ed.) Fundamentals of Interface and Colloid Science, vol. V. Academic Press, San Diego (2005)
Rezwan, K., Meier, L.P., Gauckler, L.J.: A prediction method for the isoelectric point of binary protein mixtures of bovine serum albumin and lysozyme adsorbed on colloidal titania and alumina particles. Langmuir 21, 3493–3497 (2005)
Sam, A., Gomez, C.O., Finch, J.A.: Axial velocity profiles of single bubbles in water/frother solutions. Int. J. Miner. Process. 47, 177–196 (1996)
Scales, P.J., Grieser, F., Healy, T.W., White, L.R., Chan, D.Y.C.: Electrokinetics of the silica-solution interface: a flat plate streaming potential study. Langmuir 8, 965–974 (1992)
Shahid, F., Gomez, J.E., Brinbaum, E.R., Darnall, D.W.: The lanthanide-induced N–F transition and acid expansion of serum. Albumin. J. Biol. Chem. 257, 5618–5622 (1982)
Stockelhuber, K.W.: Stability and rupture of aqueous wetting films. Eur. Phys. J. E 12, 431–435 (2003)
Todorov, R., Cohen, R., Exerowa, D.: Foam films from dipalmitoyl phosphatidylcholine (DPPC) and its natural mixtures. Colloids Surf. A 310, 32–38 (2007)
Yampolskaya, G., Platikanov, D.: Proteins at fluid interfaces: adsorption layers and thin liquid films. Adv. Colloid Interface Sci. 128–130, 159–183 (2006)
Yampolskaya, G.P., Rangelova, N.I., Bonrova, L.E., Platikanov, D.N., Izmailova, V.N.: The formation of foam black films of proteins. Biophysica 22, 939–945 (1977)
Ybert, C., di Meglio, J.-M.: Ascending air bubbles in protein solutions. Eur. Phys. J. B 4, 313–319 (1998)
Zawala, J., Drzymala, J., Malysa, K.: An investigation into the mechanism of the three-phase contact formation at fluorite surface by colliding bubble. Int. J. Miner. Process. 88, 72–79 (2008)
Zembala, M., Adamczyk, Z., Warszynski, P.: Streaming potential of mica covered by latex particles. Colloids Surf. A 222, 329–339 (2003)
Zhang, Y., Finch, J.A.: A note on single bubble motion in surfactant solutions. J. Fluid Mech. 429, 63–66 (2001)
Zhang, Y., McLaughlin, J.B., Finch, J.A.: Bubble velocity profile and model of surfactant mass transfer to bubble surface. Chem. Eng. Sci. 56, 6605–6616 (2001)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Open Access This is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
About this article
Cite this article
Zawala, J., Todorov, R., Olszewska, A. et al. Influence of pH of the BSA solutions on velocity of the rising bubbles and stability of the thin liquid films and foams. Adsorption 16, 423–435 (2010). https://doi.org/10.1007/s10450-010-9232-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10450-010-9232-3