Abstract
Stability and wetting properties changes of systems formed of phospholipid DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) layers covering silica particles or glass slides due to the phospholipase A2 (PLA2) action were determined by zeta potential measurements and the surface free energy evaluation, respectively. The comparison of the zeta potential and surface free energy, which was evaluated from advancing and receding contact angles via applying models of interfacial interactions, i.e. van Oss et al. (LWAB) and contact angle hysteresis (CAH), was found to be helpful for better understanding the mechanism of PLA2 action on the lipid layers, what is discussed in the paper.
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Blodgett, K.B., Langmuir, I.: Built-up films of barium stearate and their optical properties. Phys. Rev. 51, 964–982 (1937)
Chibowski, E.: Surface free energy of a solid from contact angle hysteresis. Adv. Colloid Interface Sci. 103, 149–172 (2003)
Chibowski, E.: Surface free energy and wettability of silyl layers on silicon determined from contact angle hysteresis. Adv. Colloid Interface Sci. 113, 121–133 (2005)
Chibowski, E.: On some relations between advancing, receding and Young’s contact angles. Adv. Colloid Interface Sci. 133, 51–59 (2007)
Chibowski, E., Holysz, L., Jurak, M.: Effect of a lipolytic enzyme on wettability and topography of phospholipid layers deposited on solid support. Colloids Surf. A: Physicochem. Eng. Asp. 321, 131–136 (2008)
Dennis, E.A.: Phospholipases. In: Dennis, E.A. (ed.) The Enzymes, pp. 307–353. Academic Press, New York (1983)
Grandbois, M., Clausen-Schaumann, H., Gaub, H.: Atomic force microscope imaging of phospholipid bilayer degradation by phospholipase A2. Biophys. J. 74, 2398–2404 (1998)
Heurtault, B., Saulnier, P., Pech, B., Proust, J.E., Benoit, J.P.: Physico-chemical stability of colloidal lipid particles. Biomaterials 24, 4283–4300 (2003)
Jørgensen, K., Davidsen, J., Mouritsen, O.G.: Biophysical mechanisms of phospholipase A2 action and their use in liposome-based drug delivery. FEBS Lett. 531, 23–27 (2002)
Langmuir, I., Schaefer, V.J.: Activities of urease and pepsin monolayers. J. Am. Chem. Soc. 57, 1007 (1938)
Moura, S.P., Carmona-Ribeiro, A.M.: Biomimetic particles: optimization of phospholipid bilayer coverage on silica and colloid stabilization. Langmuir 21, 10160–10164 (2005)
Mouritsen, O.G., Andresen, T.L., Halperin, A., Hansen, P.L., Jakobsen, A.F., Jensen, U.B., Jensen, M.Ø., Jørgensen, K., Kaasgaard, T., Leidy, C., Simonsen, A.C., Peters, G.H., Weiss, M.: Activation of interfacial enzymes at membrane surfaces. J. Phys.: Condens. Matter 18, 1293–1304 (2006)
Nielsen, L.K., Risbo, J., Callisen, T.H., Bjørnholm, T.: Lag-burst kinetics in phospholipase A2 hydrolysis of DPPC bilayers visualized by atomic force microscopy. Biochim. Biophys. Acta 1420, 266–271 (1999)
Preočanin, T., Kallay, N.: Point of zero charge and surface charge density of TiO2 in aqueous electrolyte solution as obtained by potentiometric mass titration. Croat. Chem. Acta 79, 95–106 (2006)
Rapuano, R., Carmona-Ribeiro, A.M.: Physical adsorption of bilayer membranes on silica. J. Colloid Interface Sci. 193, 104–111 (1997)
Satoh, K.: Determination of binding constants of Ca2+, Na+, and Cl− ions to liposomal membrane of dipalmitoylphosphatidylcholine at gel phase by particle electrophoresis. Biochim. Biophys. Acta 1239, 239–248 (1995)
Troutier, A.L., Ladavière, C.: An overview of lipid membrane supported by colloidal particles. Adv. Colloid Interface Sci. 133, 1–21 (2007)
van Oss, C.J., Chaudhury, M.K., Good, R.J.: Interfacial Lifshitz-van der Waals and polar interactions in macroscopic systems. Chem. Rev. 88, 927–941 (1988)
Verheij, H.M., Volwerk, J.J., Jansen, E.H., Puyk, W.C., Dijkstra, B.W., Drenth, J., de Haas, G.H.: Methylation of histidine-48 in pancreatic phospholipase A2. Role of histidine and calcium ion in the catalytic mechanism. Biochemistry 19, 743–750 (1980)
Wacklin, H.P., Tiberg, F., Fragneto, G., Thomas, R.K.: Distribution of reaction products in phospholipase A2 hydrolysis. Biochim. Biophys. Acta 1768, 1036–1049 (2007)
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Jurak, M., Chibowski, E. Zeta potential and surface free energy changes of solid-supported phospholipid (DPPC) layers caused by the enzyme phospholipase A2 (PLA2). Adsorption 15, 211–219 (2009). https://doi.org/10.1007/s10450-009-9172-y
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DOI: https://doi.org/10.1007/s10450-009-9172-y