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SMB Operation for Three-Fraction Separations: Purification of Plasmid DNA

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Abstract

The Simulated Moving Bed technology is extended to incorporate a cleaning in place step, and it is then applied by exploiting size exclusion chromatography to purify plasmid DNA. Experimental performances are discussed in the light of our theoretical understanding of the SMB behavior.

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Authors and Affiliations

  1. ETH Swiss Federal Institute of Technology Zurich, Sonneggstrasse 3, CH-8092, Zurich, Switzerland

    Galatea Paredes & Marco Mazzotti

  2. Amersham Biosciences Europe GmbH, D-79111, Freiburg, Germany

    Joachim Stadler

  3. ETH Swiss Federal Institute of Technology Zurich, Sonneggstrasse 3, CH-8092, Zurich, Switzerland

    Stefan Makart & Massimo Morbidelli

Authors
  1. Galatea Paredes
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  2. Marco Mazzotti
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  3. Joachim Stadler
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  4. Stefan Makart
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  5. Massimo Morbidelli
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Correspondence to Marco Mazzotti.

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Paredes, G., Mazzotti, M., Stadler, J. et al. SMB Operation for Three-Fraction Separations: Purification of Plasmid DNA. Adsorption 11 (Suppl 1), 841–845 (2005). https://doi.org/10.1007/s10450-005-6033-1

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  • DOI: https://doi.org/10.1007/s10450-005-6033-1

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