Acta Biotheoretica

, Volume 62, Issue 4, pp 455–478

In Silico Analysis of Acinetobacter baumannii Phospholipase D as a Subunit Vaccine Candidate

  • Elaheh Zadeh Hosseingholi
  • Iraj Rasooli
  • Seyed Latif Mousavi Gargari
Regular Article

DOI: 10.1007/s10441-014-9226-8

Cite this article as:
Zadeh Hosseingholi, E., Rasooli, I. & Mousavi Gargari, S.L. Acta Biotheor (2014) 62: 455. doi:10.1007/s10441-014-9226-8

Abstract

The rate of human health care-associated infections caused by Acinetobacter baumannii has increased significantly in recent years for its remarkable resistance to desiccation and most antibiotics. Phospholipases, capable of destroying a phospholipid substrate, are heterologous group of enzymes which are believed to be the bacterial virulence determinants. There is a need for in silico studies to identify potential vaccine candidates. A. baumannii phospholipase D (PLD) role has been proved in increasing organism’s resistance to human serum, destruction of host epithelial cell and pathogenesis in murine model. In this in silico study high potentials of A. baumannii PLD in elicitation of humoral and cellular immunities were elucidated. Thermal stability, long half-life, non-similarity to human and gut flora proteome and non-allergenicity were in a list of A. baumannii PLD positive properties. Potential epitopic sequences were also identified that could be used as peptide vaccines against A. baumannii and various other human bacterial pathogens.

Keywords

Acinetobacter baumannii In silico Phospholipase D Vaccine 

Supplementary material

10441_2014_9226_MOESM1_ESM.docx (281 kb)
Supplementary material 1 (DOCX 281 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Elaheh Zadeh Hosseingholi
    • 1
  • Iraj Rasooli
    • 1
    • 2
  • Seyed Latif Mousavi Gargari
    • 1
  1. 1.Department of BiologyShahed UniversityTehranIran
  2. 2.Molecular Microbiology Research CenterShahed UniversityTehranIran

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