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Modelling of Cells Bioenergetics

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Abstract

This paper presents an integrated model describing the control of Saccharomyces cerevisiae yeast cells bioenergetics. This model describes the oxidative and respirofermentative metabolism. The model assumes that the mitochondria of the Saccharomyces cerevisiae cells are charged with NADH during the tricarboxylic acid cycle, and NADH is discharged from mitochondria later in the electron transport system. Selected effects observed in the Saccharomyces cerevisiae eucaryotic cells, including the Pasteur's and Crabtree effects, are also modeled.

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Abbreviations

TCA:

Tricarboxylic acid

V(+)NADH :

The rate of mitochondria charging with NADH

V(-)NADH :

The rate of NADH discharging from mitochondria

fATP:

Positive feedback for ATP

cycle(n) :

The nth DOC oscillation cycle

portion(n) :

The nth glucose portion

DOC:

Dissolved O2 concentration in the culture medium

DOCset :

The set value of DOC

RQ:

Respiratory quotient

RQset :

The set value of RQ

RQ0 :

The respiratory quotient when no ethanol is being produced

RQmax :

The maximum value of respiratory quotient in the current DOC oscillation cycle

RQmax(n) :

The maximum value of respiratory quotient in the nth DOC oscillation cycle

MaxGluCon:

The maximum value of glucose concentration in the culture medium in the DOC oscillation cycle

GluCon:

The glucose concentration in the culture medium

GluConcritical :

The critical value of glucose concentration, denotes such glucose concentration in the culture medium which, if exceeded, causes fermentation in good aerobic conditions

mtNADHmin :

The minimal level of intramitochondrial NADH

mtNADHnormal :

The normal level of intramitochondrial NADH

mtNADHcritical :

The critical level of intramitochondrial NADH

EPR:

The ethanol production rate

OUR:

The oxygen uptake rate

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Acknowledgments

I am grateful to Prof. Tadeusz Lachowicz at the University of Wroclaw, Poland and Prof. Mykhailo V. Gonchar from the Institute of Cell Biology, NAS, Ukraine for their help and critical reading of this article. I am also sincerely indebted to Prof. Tadeusz Miskiewicz and Ms Alicja Stempniak from the Department of Bioprocess Engineering, Wroclaw University of Economics, Poland, for their help in the preparation and execution of my experiments.

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  1. Faculty of Mathematics, Computer Science and Econometrics, University of Zielona Gora, The BTF, Szafrana 4a, 65-516, Zielona Gora, Poland

    Andrzej Kasperski

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Correspondence to Andrzej Kasperski.

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Kasperski, A. Modelling of Cells Bioenergetics. Acta Biotheor 56, 233–247 (2008). https://doi.org/10.1007/s10441-008-9050-0

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