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In Vitro and In Silico Characterization of the Aggregation of Thrombi on Textured Ventricular Cannula

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Abstract

The unacceptably high stroke rate associated with HeartMate 3 ventricular assist device (VAD) without signs of adherent pump thrombosis is hypothesized to be the result of the emboli produced by the inflow cannula, that are ingested and ejected from the pump. This in vitro and numerical study aimed to emulate the surface features and supraphysiological shear of a ventricular cannula to provide insight into their effect on thrombogenesis. Human whole blood was perfused at calibrated flow rates in a microfluidic channel to achieve shear rates 1000–7500 s−1, comparable to that experienced on the cannula. The channel contained periodic teeth representative of the rough sintered surface of the HeartMate 3 cannula. The deposition of fluorescently labeled platelets was visualized in real time and analyzed with a custom entity tracking algorithm. Numerical simulations of a multi-constituent thrombosis model were performed to simulate laminar blood flow in the channel. The sustained growth of adherent platelets was observed in all shear conditions (\(p<\) 0.05). However, the greatest deposition was observed at the lower shear rates. The location of deposition with respect to the microfluidic teeth was also found to vary with shear rate. This was confirmed by CFD simulation. The entity tracking algorithm revealed the spatial variation of instances of embolic events. This result suggests that the sintered surface of the ventricular cannula may engender unstable thrombi with a greater likelihood of embolization at supraphysiological shear rates.

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Acknowledgements

We would like to extend our gratitude to the National Institute of Health Grant HL089456 for funding this study.

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Authors and Affiliations

  1. Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY, 14853, USA

    Wenxuan He & Junhyuk Kang

  2. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, 14853, USA

    Abhishek Karmakar, Grant Rowlands, Samuel Schirmacher, Rodrigo Méndez-Rojano & James Antaki

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  1. Wenxuan He
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  2. Abhishek Karmakar
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Correspondence to James Antaki.

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He, W., Karmakar, A., Kang, J. et al. In Vitro and In Silico Characterization of the Aggregation of Thrombi on Textured Ventricular Cannula. Ann Biomed Eng (2024). https://doi.org/10.1007/s10439-024-03504-1

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