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Fibrin Hydrogel Layer-Anchored Pericardial Matrix Prevents Epicardial Adhesion in the Severe Heart Adhesion-Induced Miniature Pig Model

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Abstract

Postoperative adhesion is a very common and serious complication that occurs frequently in cardiac surgery. The purpose of this study was to evaluate the efficacy of a fibrin hydrogel layer-anchored decellularized pericardial matrix in preventing pericardial adhesions in a miniature pig model with a myocardial injury. Fibrin hydrogel layer-anchored decellularized pericardial matrix was prepared by spraying a mixture of fibrinogen and thrombin on a fibrinogen-doped decellularized pericardium. Cardiac injury was generated by abrading and desiccating the epicardial surface of a miniature pig to induce severe postoperative adhesions. The adhesion between the epicardial surface and fibrin hydrogel layer-anchored decellularized pericardial matrix in three different regions (left outer, front, and right outer) was evaluated macroscopically one month after surgery. The fibrin hydrogel layer-anchored decellularized pericardial matrix showed significantly less adhesion than an autologous pericardium (0.2 ± 0.7 in DPM-FHG0.5 and 0.4 ± 0.8 in DPM-FHG1, p < 0.01) and expanded polytetrafluoroethylene (ePTFE) (1.6 ± 0.5, p < 0.05). The fibrin hydrogel concentration had no effect on preventing postoperative adhesion. A thinner fibrin hydrogel layer was observed on the decellularized pericardial matrix one month after surgery; however, the inside of the matrix was filled with fibrin hydrogel. Fibrin hydrogel layer-anchored decellularized pericardial matrix prevented postoperative epicardial adhesions in a miniature pig model. Our findings suggest that pericardial closure using a fibrin hydrogel layer-anchored decellularized pericardial matrix is a promising method for preventing adverse outcomes in reoperative surgeries.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgments

The Division of Acellular Tissue and Regenerative Medical Materials is an endowment department, established with a grant from ADEKA Corporation. This work was supported by funding from the ADEKA Corporation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank KM Biologics Co., Ltd. for providing the fibrin glue (Bolheal®), which is commercially available. We thank Ashleigh Cooper, PhD, from Edanz (www.edanzediting.com/ac) for editing a draft of this manuscript.

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Author notes

  1. Yoshihide Hashimoto and Akitatsu Yamashita have equally contributed to this work.

Authors and Affiliations

  1. Endowed Division of Acellular Tissue and Regenerative Medical Materials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-0062, Japan

    Yoshihide Hashimoto, Akitatsu Yamashita, Yongwei Zhang & Seiichi Funamoto

  2. Department of Material-Based Medical Engineering, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-0062, Japan

    Yoshihide Hashimoto, Akitatsu Yamashita, Masaki Tabuchi, Yongwei Zhang, Seiichi Funamoto & Akio Kishida

  3. Yamashita Clinic-Nishiya, 1083 Nishiyamachi, Hodogaya-ku, Yokohama, Kanagawa, 240-0052, Japan

    Akitatsu Yamashita

  4. Department of Cardiovascular Surgery, Sumitomo Hospital, 5-3-20 Nakanoshima, Kita-ku, Osaka, 530-0005, Japan

    Masaki Tabuchi

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  1. Yoshihide Hashimoto
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Correspondence to Seiichi Funamoto.

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Yoshihide Hashimoto, Akitatsu Yamashita, Yongwei Zhang, Seiichi Funamoto, and Akio Kishida receive a research support from ADEKA Corporation. Masaki Tabuchi has no conflict of interest.

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Hashimoto, Y., Yamashita, A., Tabuchi, M. et al. Fibrin Hydrogel Layer-Anchored Pericardial Matrix Prevents Epicardial Adhesion in the Severe Heart Adhesion-Induced Miniature Pig Model. Ann Biomed Eng 52, 282–291 (2024). https://doi.org/10.1007/s10439-023-03373-0

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