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Characterization of Chitosan-Based Scaffolds Seeded with Sheep Nasal Chondrocytes for Cartilage Tissue Engineering

Annals of Biomedical Engineering volume 49, pages 1572–1586 (2021)Cite this article

Abstract

The treatment of cartilage defect remains a challenging issue in clinical practice. Chitosan-based materials have been recognized as a suitable microenvironment for chondrocyte adhesion, proliferation and differentiation forming articular cartilage. The use of nasal chondrocytes to culture articular cartilage on an appropriate scaffold emerged as a promising novel strategy for cartilage regeneration. Beside excellent properties, chitosan lacks in biological activity, such as RGD-sequences. In this work, we have prepared pure and protein-modified chitosan scaffolds of different deacetylation degree and molecular weight as platforms for the culture of sheep nasal chondrocytes. Fibronectin (FN) was chosen as an adhesive protein for the improvement of chitosan bioactivity. Prepared scaffolds were characterised in terms of microstructure, physical and biodegradation properties, while FN interactions with different chitosans were investigated through adsorption–desorption studies. The results indicated faster enzymatic degradation of chitosan scaffolds with lower deacetylation degree, while better FN interactions with material were achieved on chitosan with higher number of amine groups. Histological and immunohistochemical analysis of in vitro engineered cartilage grafts showed presence of hyaline cartilage produced by nasal chondrocytes.

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Acknowledgment

This work has been supported by the Croatian Science Foundation under the project IP-2014-09-3752, the European Union’s Horizon 2020 research and innovation program under grant agreement No 681103, BioChip.

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Authors and Affiliations

  1. Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, p.p.177, 10001, Zagreb, Croatia

    Anamarija Rogina, Lucija Štefan, Marica Ivanković & Hrvoje Ivanković

  2. Faculty of Science, University of Zagreb, Horvatovac102a, 10001, Zagreb, Croatia

    Maja Pušić & Inga Urlić

  3. Department of Histology and Embryology, School of Medicine, University of Zagreb, Šalata 3, 10001, Zagreb, Croatia

    Alan Ivković

  4. Department of Orthopaedic Surgery, University Hospital Sveti Duh, Sveti Duh 64, 10001, Zagreb, Croatia

    Alan Ivković

  5. Department of Biotechnology, University of Rijeka, Radmile Matejčić 2, 51000, Rijeka, Croatia

    Alan Ivković

  6. University of Applied Health Sciences, Mlinarska cesta 38, 10001, Zagreb, Croatia

    Alan Ivković

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  1. Anamarija Rogina
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Rogina, A., Pušić, M., Štefan, L. et al. Characterization of Chitosan-Based Scaffolds Seeded with Sheep Nasal Chondrocytes for Cartilage Tissue Engineering. Ann Biomed Eng 49, 1572–1586 (2021). https://doi.org/10.1007/s10439-020-02712-9

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Keywords

  • Chitosan
  • Biodegradation
  • Fibronectin
  • Nasal chondrocytes
  • Hyaline cartilage