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Hyperelastic Mechanical Properties of Ex Vivo Normal and Intrauterine Growth Restricted Placenta

Annals of Biomedical Engineering volume 46, pages 1066–1077 (2018)Cite this article

Abstract

Intrauterine Growth Restriction (IUGR) is a serious and prevalent pregnancy complication that is due to placental insufficiency and IUGR babies suffer significantly higher risks of mortality and morbidity. Current detection rate for IUGR is generally poor and thus an alternative diagnostic tool is needed to improve the IUGR detection. Elastography, a non-invasive method that measures the tissue stiffness, has been proposed as one such technique. However, to date, we have limited information on the mechanical properties of IUGR placenta. In this study, we investigated the mechanical properties of normal and IUGR placentae and prescribed a suitable hyperelastic model to describe their mechanical behaviors. A total of 46 normal and 43 IUGR placenta samples were investigated. Results showed that placenta samples were isotropic, but had a high spatial variability of stiffness. The samples also had significant viscoelasticity. IUGR placenta was observed to be slightly stiffer than normal placenta but the difference was significant only at compression rate of 0.25 Hz and with 20% compression depth. Three simple hyperelastic models—Yeoh, Ogden and Fung models, were found to be able to fit the experimentally measured mechanical behaviors, and Fung model performed slightly better. These results may be useful for optimizing placenta elastography for the detection of IUGR.

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Acknowledgments

This research was supported by the National University of Singapore Young Investigator Award 2015 (PI: Yap) and the Ministry of Education of Singapore Academic Research Fund Tier 1 Grant entitled “Placenta Blood Oxygen Monitor for Intrauterine Growth Restriction Pregnancies (PI: Yap). We would like to express our gratitude to Cecille Arquillo Laureano, Cynthia Pamela Zapata Tagarino, Maylene Zipagan and other staff members from Obstetrics & Gynecology Department at National University Hospital for their assistance with patient eligibility and tissue procurement.

Conflict of interest

All authors have no conflict of interest to declare.

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Affiliations

  1. Department of Biomedical Engineering, National University of Singapore, 9 Engineering Drive 1, #02-04, Singapore, 117575, Singapore

    Shier Nee Saw, Jess Yi Ru Low, May Han Huang Ong, Yu Wei Poh & Choon Hwai Yap

  2. Department of Obstetrics and Gynecology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health Systems, Singapore, Singapore

    Citra Nurfarah Zaini Mattar & Arijit Biswas

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  1. Shier Nee Saw
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  2. Jess Yi Ru Low
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Correspondence to Choon Hwai Yap.

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Associate Editor Eiji Tanaka oversaw the review of this article.

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Saw, S.N., Low, J.Y.R., Ong, M.H.H. et al. Hyperelastic Mechanical Properties of Ex Vivo Normal and Intrauterine Growth Restricted Placenta. Ann Biomed Eng 46, 1066–1077 (2018). https://doi.org/10.1007/s10439-018-2019-5

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  • DOI: https://doi.org/10.1007/s10439-018-2019-5

Keywords

  • Placenta mechanical properties
  • Intrauterine growth restriction
  • Fetal growth restriction
  • 3D hyperelastic model
  • Uniaxial mechanical testing