A Theoretical Study on Inhibition of Melanoma with Controlled and Targeted Delivery of siRNA via Skin Using SPACE-EGF
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Melanoma is a potentially lethal skin cancer with high mortality rate. Recently, the peptide-mediated transdermal delivery of small interference RNA (siRNA) emerges as a promising strategy to treat melanoma by inducing the apoptosis of tumor cells, but the related theoretical model describing the delivery of siRNA under the effect of SPACE-EGF, the growth inhibition of melanoma and the dynamic expanding of the bump on the skin due to the growth of melanoma has not been reported yet. In this article, a theoretical model is developed to describe the percutaneous siRNA delivery mediated by SPACE-EGF to melanoma and the growth inhibition of melanoma. The results present the spatial–temporal distribution of siRNA and the growth of melanoma under the inhibition of siRNA, which shows a good consistency with the experimental results. In addition, this model represents the uplift process of tumors on the skin surface. The model presented here is a useful tool to understand the whole process of the SPACE-EGF-mediated delivery of the siRNA to melanoma through skin, to predict the therapeutic effect, and to optimize the therapeutic strategy, providing valuable references for the treatment of melanoma.
KeywordsMelanoma siRNA Peptide carrier Tumor growth inhibition Transdermal drug delivery
This work was supported partly by the Specialized Research Fund for the Doctoral Program of Higher Education of China (20133402120033), the National Natural Science Foundation of China (81571768, 81627806), and the Natural Science Foundation of Anhui Province (1408085ME96). We would like to thank Research Center for Life Sciences at the University of Science and Technology of China for assistance.
J.L. and W.D. developed the theoretical model. J.L. accomplished the numerical simulation and data analysis. R.R, W.D., M.C. and L.W. designed the experiments. R.R., L.Z., M.C. and P.W. conducted the experiments. J.L. wrote the manuscript with inputs from all co-authors. All authors reviewed the manuscript.
Conflict of interest
The authors declare no competing financial interests.
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