Mechanical properties of the cardiac tissue play an important role in normal heart function. The goal of this study was to determine the passive mechanical properties of all heart chambers through a paired comparison study in an ovine model. Ovine heart was used due its physiological and anatomical similarities to human heart. A total of 189 specimens from anterior and posterior portions of the left and right ventricles, atria, and appendages underwent biaxial mechanical testing. A Fung-type strain energy function was used to fit the experimental data. Tissue behavior was quantified based on the magnitude of strain energy, as indicator of tissue stiffness, at equibiaxial strains of 0.10, 0.15, and 0.20. Statistical analysis revealed no significant difference in strain energy storage between anterior and posterior portions of each chamber, except for the right ventricle where strain energy storage in the posterior specimens were higher than the anterior specimens. Additionally, all chambers from the left side of the heart had significantly higher strain energy storage than the corresponding chambers on the right side. Furthermore, the highest to lowest stored strain energy were associated with ventricles, appendages, and atria, respectively. Microstructure of tissue specimens from different chambers was also compared using histology.
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Left atrial appendage
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This work was supported by the Knoebel Center for the Study of Aging and Professional Research Opportunity Funds administered by University of Denver (Grant No. 142235).
Conflict of interest
The authors have no conflict of interest to declare.
Associate Editor Ellen Kuhl oversaw the review of this article.
The individual coefficient sets of the four parameter Fung exponential constitutive model for all of the individual hearts (n = 19). Furthermore, the maximum Green strain values reached in the biaxial tests were shown in the table.
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Javani, S., Gordon, M. & Azadani, A.N. Biomechanical Properties and Microstructure of Heart Chambers: A Paired Comparison Study in an Ovine Model. Ann Biomed Eng 44, 3266–3283 (2016). https://doi.org/10.1007/s10439-016-1658-7
- Cardiac mechanics
- Passive mechanical behavior
- Biaxial testing
- Atrial appendage