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3D Printing of Tissue Engineered Constructs for In Vitro Modeling of Disease Progression and Drug Screening

Annals of Biomedical Engineering volume 45, pages 164–179 (2017)Cite this article

Abstract

2D cell culture and preclinical animal models have traditionally been implemented for investigating the underlying cellular mechanisms of human disease progression. However, the increasing significance of 3D vs. 2D cell culture has initiated a new era in cell culture research in which 3D in vitro models are emerging as a bridge between traditional 2D cell culture and in vivo animal models. Additive manufacturing (AM, also known as 3D printing), defined as the layer-by-layer fabrication of parts directed by digital information from a 3D computer-aided design file, offers the advantages of simultaneous rapid prototyping and biofunctionalization as well as the precise placement of cells and extracellular matrix with high resolution. In this review, we highlight recent advances in 3D printing of tissue engineered constructs that recapitulate the physical and cellular properties of the tissue microenvironment for investigating mechanisms of disease progression and for screening drugs.

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Acknowledgments

Research reported in this publication was supported in part by the National Cancer Institute of the National Institutes of Health under award number R01CA163499. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Vanderbilt University, PMB 351604, 2301 Vanderbilt Place, Nashville, TN, 37232, USA

    Joseph Vanderburgh & Scott A. Guelcher

  2. Department of Veterans Affairs, Tennessee Valley Healthcare System, 1235 MRB IV, 2222 Pierce Ave, Nashville, TN, 37232, USA

    Julie A. Sterling

  3. Center for Bone Biology, Vanderbilt University Medical Center, Nashville, TN, USA

    Julie A. Sterling & Scott A. Guelcher

  4. Division of Clinical Pharmacology, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA

    Julie A. Sterling

  5. Department of Cancer Biology, Vanderbilt University, Nashville, TN, USA

    Julie A. Sterling

  6. Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA

    Scott A. Guelcher

Authors
  1. Joseph Vanderburgh
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  2. Julie A. Sterling
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  3. Scott A. Guelcher
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Corresponding author

Correspondence to Scott A. Guelcher.

Additional information

Associate Editor Jos Malda oversaw the review of this article.

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Vanderburgh, J., Sterling, J.A. & Guelcher, S.A. 3D Printing of Tissue Engineered Constructs for In Vitro Modeling of Disease Progression and Drug Screening. Ann Biomed Eng 45, 164–179 (2017). https://doi.org/10.1007/s10439-016-1640-4

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  • DOI: https://doi.org/10.1007/s10439-016-1640-4

Keywords

  • Additive Manufacturing
  • 3D printing
  • Bioprinting
  • Drug screening
  • Tissue engineered constructs