Annals of Biomedical Engineering

, Volume 45, Issue 1, pp 273–285

Thiol-ene Clickable Poly(glycidol) Hydrogels for Biofabrication

  • Simone Stichler
  • Tomasz Jungst
  • Martha Schamel
  • Ilona Zilkowski
  • Matthias Kuhlmann
  • Thomas Böck
  • Torsten Blunk
  • Jörg Teßmar
  • Jürgen Groll
Additive Manufacturing of Biomaterials, Tissues, and Organs

DOI: 10.1007/s10439-016-1633-3

Cite this article as:
Stichler, S., Jungst, T., Schamel, M. et al. Ann Biomed Eng (2017) 45: 273. doi:10.1007/s10439-016-1633-3

Abstract

In this study we introduce linear poly(glycidol) (PG), a structural analog of poly(ethylene glycol) bearing side chains at each repeating unit, as polymer basis for bioink development. We prepare allyl- and thiol-functional linear PG that can rapidly be polymerized to a three-dimensionally cross-linked hydrogel network via UV mediated thiol-ene click reaction. Influence of polymer concentration and UV irradiation on mechanical properties and swelling behavior was examined. Thiol-functional PG was synthesized in two structural variations, one containing ester groups that are susceptible to hydrolytic cleavage, and the other one ester-free and stable against hydrolysis. This allowed the preparation of degradable and non-degradable hydrogels. Cytocompatibility of the hydrogel was demonstrated by encapsulation of human bone marrow-derived mesenchymal stem cells (hBMSCs). Rheological properties of the hydrogels were adjusted for dispense plotting by addition of high molecular weight hyaluronic acid. The optimized formulation enabled highly reproducible plotting of constructs composed of 20 layers with an overall height of 3.90 mm.

Keywords

Bioink Bioprinting Dispense plotting Poly(glycidol) Hyaluronic acid 

Supplementary material

10439_2016_1633_MOESM1_ESM.docx (1001 kb)
Supplementary material 1 (DOCX 1001 kb)

Supplementary material 2 (MP4 11037 kb)

Supplementary material 3 (MP4 12577 kb)

Funding information

Funder NameGrant NumberFunding Note
Seventh Framework Programme
  • 309962
izkf würzburg
  • D-219

Copyright information

© Biomedical Engineering Society 2016

Authors and Affiliations

  • Simone Stichler
    • 1
  • Tomasz Jungst
    • 1
  • Martha Schamel
    • 1
  • Ilona Zilkowski
    • 1
  • Matthias Kuhlmann
    • 1
  • Thomas Böck
    • 2
  • Torsten Blunk
    • 2
  • Jörg Teßmar
    • 1
  • Jürgen Groll
    • 1
  1. 1.Department for Functional Materials in Medicine and DentistryUniversity of WürzburgWürzburgGermany
  2. 2.Department of Trauma, Hand, Plastic and Reconstructive SurgeryUniversity of WürzburgWürzburgGermany

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