Thiol-ene Clickable Poly(glycidol) Hydrogels for Biofabrication
- 1k Downloads
In this study we introduce linear poly(glycidol) (PG), a structural analog of poly(ethylene glycol) bearing side chains at each repeating unit, as polymer basis for bioink development. We prepare allyl- and thiol-functional linear PG that can rapidly be polymerized to a three-dimensionally cross-linked hydrogel network via UV mediated thiol-ene click reaction. Influence of polymer concentration and UV irradiation on mechanical properties and swelling behavior was examined. Thiol-functional PG was synthesized in two structural variations, one containing ester groups that are susceptible to hydrolytic cleavage, and the other one ester-free and stable against hydrolysis. This allowed the preparation of degradable and non-degradable hydrogels. Cytocompatibility of the hydrogel was demonstrated by encapsulation of human bone marrow-derived mesenchymal stem cells (hBMSCs). Rheological properties of the hydrogels were adjusted for dispense plotting by addition of high molecular weight hyaluronic acid. The optimized formulation enabled highly reproducible plotting of constructs composed of 20 layers with an overall height of 3.90 mm.
KeywordsBioink Bioprinting Dispense plotting Poly(glycidol) Hyaluronic acid
The research leading to these results has received funding from the European Union’s Seventh Framework Programme (FP7/2007–2013) under Grant Agreement n° 309962 (Project HydroZONES) and from the Interdisciplinary Center for Clinical Research Würzburg (Project Number D-219).
Supplementary material 2 (MP4 11037 kb)
Supplementary material 3 (MP4 12577 kb)
- 8.Fitton, A. O., J. Hill, D. E. Jane, and R. Millar. Synthesis of simple oxetanes carrying reactive 2-substituents. Synthesis 1140–1142:1987, 1987.Google Scholar
- 10.Groll, J., T. Boland, T. Blunk, J. A. Burdick, D. W. Cho, P. D. Dalton, B. Derby, G. Forgacs, Q. Li, V. A. Mironov, L. Moroni, M. Nakamura, W. Shu, S. Takeuchi, G. Vozzi, T. B. F. Woodfield, T. Xu, J. J. Yoo, and J. Malda. Biofabrication: reappraising the definition of an evolving field. Biofabrication 8:013001, 2016.CrossRefPubMedGoogle Scholar
- 25.Pescosolido, L., W. Schuurman, J. Malda, P. Matricardi, F. Alhaique, T. Coviello, P. R. van Weeren, W. J. A. Dhert, W. E. Hennink, and T. Vermonden. Hyaluronic acid and dextran-based semi-IPN hydrogels as biomaterials for bioprinting. Biomacromolecules 12:1831–1838, 2011.CrossRefPubMedGoogle Scholar
- 28.Schindelin, J., I. Arganda-Carreras, E. Frise, V. Kaynig, M. Longair, T. Pietzsch, S. Preibisch, C. Rueden, S. Saalfeld, B. Schmid, J.-Y. Tinevez, D. J. White, V. Hartenstein, K. Eliceiri, P. Tomancak, and A. Cardona. Fiji: an open-source platform for biological-image analysis. Nat. Methods 7:676–682, 2012.CrossRefGoogle Scholar
- 29.Schuurman, W., P. A. Levett, M. W. Pot, P. R. van Weeren, W. J. A. Dhert, D. W. Hutmacher, F. P. W. Melchels, T. J. Klein, and J. Malda. Gelatin-methacrylamide hydrogels as potential biomaterials for fabrication of tissue-engineered cartilage constructs. Macromol. Biosci. 13:551–561, 2013.CrossRefPubMedGoogle Scholar