Tracking of Drug Release and Material Fate for Naturally Derived Omega-3 Fatty Acid Biomaterials
In vitro and in vivo studies were conducted on omega-3 fatty acid-derived biomaterials to determine their utility as an implantable material for adhesion prevention following soft tissue hernia repair and as a means to allow for the local delivery of antimicrobial or antibiofilm agents. Naturally derived biomaterials offer several advantages over synthetic materials in the field of medical device development. These advantages include enhanced biocompatibility, elimination of risks posed by the presence of toxic catalysts and chemical crosslinking agents, and derivation from renewable resources. Omega-3 fatty acids are readily available from fish and plant sources and can be used to create implantable biomaterials either as a stand-alone device or as a device coating that can be utilized in local drug delivery applications. In-depth characterization of material erosion degradation over time using non-destructive imaging and chemical characterization techniques provided mechanistic insight into material structure: function relationship. This in turn guided rational tailoring of the material based on varying fatty acid composition to control material residence time and hence drug release. These studies demonstrate the utility of omega-3 fatty acid derived biomaterials as an absorbable material for soft tissue hernia repair and drug delivery applications.
KeywordsFish oil Omega-3 fatty acids In vivo material tracking IVIS Fluorescence Biomaterials
The authors would like to thank Irina Kozlova and Anthony Horner for technical support during these studies. ERE was funded in part by grants from the National Institutes of Health (R01 GM 49039).
This work was performed using a gifted grant provided by Atrium Medical Corporation.
- 5.diZerega, G. Peritoneal Surgery, Vol. 1. New York: Springer, 1999.Google Scholar
- 7.Gunstone, Frank D. Fatty Acid and Lipid Chemistry. Gaithersburg: Aspen Publishers, Inc., 1999.Google Scholar
- 10.ISO. ISO 10993-18:2005, Chemical Characterization of Materials. In: Standardization IOf, editor, 2005.Google Scholar
- 12.Lastowka, A., G. J. Maffia, and E. M. Brown. A comparison of chemical, physical and enzymatic crosslinking of bovine type I collagen fibrils. JALCA 100:196–202, 2005.Google Scholar
- 14.Ratner, B. D., A. S. Hoffman, F. J. Schoen, and J. E. Lemons. Biomaterials Medicine an Introduction to Materials in Medicine (3rd ed.). Boston: Elsevier, 2013.Google Scholar