We report an inkjet-printed microscale magnetic structure that can be integrated on regular glass slides for the immunomagnetic screening of rare circulating tumor cells (CTCs). CTCs detach from the primary tumor site, circulate with the bloodstream, and initiate the cancer metastasis process. Therefore, a liquid biopsy in the form of capturing and analyzing CTCs may provide key information for cancer prognosis and diagnosis. Inkjet printing technology provides a non-contact, layer-by-layer and mask-less approach to deposit defined magnetic patterns on an arbitrary substrate. Such thin film patterns, when placed in an external magnetic field, significantly enhance the attractive force in the near-field close to the CTCs to facilitate the separation. We demonstrated the efficacy of the inkjet-print micromagnet array integrated immunomagnetic assay in separating COLO205 (human colorectal cancer cell line) from whole blood samples. The micromagnets increased the capture efficiency by 26% compared with using plain glass slide as the substrate.
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We thank our collaborator Professor Konstantin V. Sokolov at the University of Texas MD Anderson Cancer Center and Dr. Zhigang Li at the Geisel School of Medicine at Dartmouth for the supports in validating the presented results. We thank Ms. Nancy Lane and Drs. Michael Huebschman, Jonathan W. Uhr, and Eugene P. Frenkel at the University of Texas Southwestern Medical Center for their invaluable suggestions on the experiment design. We appreciate the help from Professor Tim Yeh and Dr. Judy Obliosca at the University of Texas at Austin with the spectrofluorometer measurement. We are grateful for the financial support from the National Institute of Health (NIH) National Cancer Institute (NCI) Cancer Diagnosis Program under Grant 1R01CA139070.
Associate Editor Jennifer West oversaw the review of this article.
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Chen, P., Huang, Y., Bhave, G. et al. Inkjet-Print Micromagnet Array on Glass Slides for Immunomagnetic Enrichment of Circulating Tumor Cells. Ann Biomed Eng 44, 1710–1720 (2016). https://doi.org/10.1007/s10439-015-1427-z
- Inkjet printing
- Circulating tumor cells
- Immunomagnetic cell separation